Kyle R Pomraning, Ziyu Dai, Nathalie Munoz, Young-Mo Kim, Yuqian Gao, Shuang Deng, Teresa Lemmon, Marie S Swita, Jeremy D Zucker, Joonhoon Kim, Stephen J Mondo, Ellen Panisko, Meagan C Burnet, Bobbie-Jo M Webb-Robertson, Beth Hofstad, Scott E Baker, Kristin E Burnum-Johnson, Jon K Magnuson
The global regulator LaeA controls secondary metabolism in diverse Aspergillus species. Here we explored its role in regulation of itaconic acid production in Aspergillus pseudoterreus . To understand its role in regulating metabolism, we deleted and overexpressed laeA, and assessed the transcriptome, proteome, and secreted metabolome prior to and during initiation of phosphate limitation induced itaconic acid production. We found that secondary metabolite clusters, including the itaconic acid biosynthetic gene cluster, are regulated by laeA and that laeA is required for high yield production of itaconic acid...
December 2022: Metabolic Engineering Communications