Renata R Nascimento, Cristhyane C Aquino, José K Sousa, Kalinne L Gadelha, Aurilene G Cajado, Carolina S Schiebel, Sarah A Dooley, Paulo A Sousa, Jefferson A Rocha, Jand R Medeiros, Pedro C Magalhães, Daniele Maria-Ferreira, Marcelo B Gois, Roberto C P Lima-Junior, Deysi V T Wong, Aldo M Lima, Amy C Engevik, Lucas D Nicolau, Mariana L Vale
Studies have reported the occurrence of gastrointestinal (GI) symptoms, primarily diarrhea in COVID-19. However, the pathobiology regarding COVID-19 in the GI tract remains limited. This work aimed to evaluate SARS-CoV-2 Spike protein interaction with gut lumen in different experimental approaches. Here, we present a novel experimental model with the inoculation of viral protein in the murine jejunal lumen, in vitro approach with human enterocytes and molecular docking analysis. Spike protein led to increased intestinal fluid accompanied by Cl- secretion, followed by intestinal edema, leukocyte infiltration, reduced glutathione levels, and increased cytokine levels (IL-6, TNF-α, IL-1β, IL-10), indicating inflammation...
March 28, 2024: Mucosal Immunology