Wentao Yan, Sheela George, Upinder Fotadar, Natalia Tyhovych, Angela Kamer, Michael J Yost, Robert L Price, Charles R Haggart, Jeffrey W Holmes, Louis Terracio
Loss of skeletal muscle profoundly affects the health and well-being of patients, and there currently is no way to replace lost muscle. We believe that a key step in the development of a prosthesis for reconstruction of dysfunctional muscular tissue is the ability to reconstitute the in vivo-like 3-dimensional (3D) organization of skeletal muscle in vitro with isolated satellite cells. In our present proof of principle studies, we have successfully constructed a multilayered culture of skeletal muscle cells, derived from neonatal satellite cells, that are distributed in a 3D pattern of organization that mimics many of the features of intact tissue...
November 2007: Tissue Engineering