Li Jiang, Yue Liu, Zhifeng Wen, Yingjun Yang, Stacy D Singer, Dennis Bennett, Wenying Xu, Zhen Su, Zhifang Yu, Josh Cohn, Xi Luo, Zhongchi Liu, Hyunsook Chae, Qiudeng Que, Zongrang Liu
Insulators in vertebrates play a role in genome architecture and orchestrate temporo-spatial enhancer-promoter interactions. In plants, insulators and their associated binding factors have not been documented as of yet, largely as a result of a lack of characterized insulators. In this study, we took a comprehensive strategy to identify and validate the enhancer-blocking insulator CW198. We show that a 1.08-kb CW198 fragment from Arabidopsis can, when interposed between an enhancer and a promoter, efficiently abrogate the activation function of both constitutive and floral organ-specific enhancers in transgenic Arabidopsis and tobacco plants...
September 2, 2022: Transgenic Research