Journals Journal of Steroid Biochemistr...

Journal of Steroid Biochemistry and Molecular Biology
Ajna Hamidovic, Soojeong Cho, John Davis
Gamma-aminobutyric acid A (GABA-A) receptors in the cells of the immune system enhance anti-inflammatory responses by regulating cytokine secretion, cytotoxic responses, and cell activation. In the CNS, the formation of GABA-A subunits into a pentameric structure has been extensively studied; however, no such study has been conducted in the immune system. The objective of the present study was to examine associations between the levels of steroid hormones and GABA-A receptor δ subunit expression in the immune system...
April 16, 2024: Journal of Steroid Biochemistry and Molecular Biology
Manal A Abbas, Aya Y Al-Kabariti, Chris Sutton
G protein-coupled estrogen receptor (GPER) plays a prominent role in facilitating the rapid, non-genomic signaling of estrogens in breast cancer cells. Herein, a comprehensive overview of the role of GPER in ER-ɑ-negative breast cancer is provided. Activation of GPER affected proliferation, metastasis and epithelial mesenchymal transition in ER-ɑ negative breast cancer cells. Clinical studies have demonstrated that GPER positivity was strongly correlated with larger tumor size and advanced clinical stage, suggesting that GPER/ERK signaling may play a role in promoting tumor progression...
April 16, 2024: Journal of Steroid Biochemistry and Molecular Biology
Jing Huang, Chao Sun, Zhuo Huang, Yong Zhu, Shi Xi Chen
Increased cortisol levels in the preovulatory follicular fluid suggests a role of glucocorticoid in human ovulation. However, the mechanisms through which cortisol regulates the ovulatory process remain poorly understood. In this study, we examined the upregulation of f5 mRNA by glucocorticoid and its receptor (Gr) in the preovulatory follicles of zebrafish. Our findings demonstrate a significant increase in 11β-hydroxysteroid dehydrogenase type 2 (hsd11b2), a cortisol response gene, in preovulatory follicles...
April 15, 2024: Journal of Steroid Biochemistry and Molecular Biology
Andrew J McGovern, Maria Angeles Arevalo, Sergio Ciordia, Luis Miguel Garcia-Segura, George E Barreto
Gonadal hormone deprivation (GHD) and decline such as menopause and bilateral oophorectomy are associated with an increased risk of neurodegeneration. Yet, hormone therapies (HTs) show varying efficacy, influenced by factors such as sex, drug type, and timing of treatment relative to hormone decline. We hypothesize that the molecular environment of the brain undergoes a transition following GHD, impacting the effectiveness of HTs. Using a GHD model in mice treated with Tibolone, we conducted proteomic analysis and identified a reprogrammed response to Tibolone, a compound that stimulates estrogenic, progestogenic, and androgenic pathways...
April 13, 2024: Journal of Steroid Biochemistry and Molecular Biology
Yu Chun Teng, Marie Claire Gielen, Nina M De Gruijter, Coziana Ciurtin, Elizabeth C Rosser, Kersti Karu
Phytosterols are lipophilic compounds found in plants with structural similarity to mammalian cholesterol. They cannot be endogenously produced by mammals and therefore always originate from diet. There has been increased interest in dietary phytosterols over the last few decades due to their association with a variety of beneficial health effects including low-density lipoprotein cholesterol lowering, anti-inflammatory and anti-cancerous effects. They are proposed as potential moderators for diseases associated with the central nervous system where cholesterol homeostasis is found to be imperative (multiple sclerosis, dementia, etc...
April 11, 2024: Journal of Steroid Biochemistry and Molecular Biology
Xiao Yin Lee, Wout Van Eynde, Christine Helsen, Hanne Willems, Kaat Peperstraete, Sofie De Block, Arnout Voet, Frank Claessens
The androgen receptor (AR) is a steroid activated transcription factor which recognizes DNA motifs resembling inverted repeats of a conserved 5'-AGAACA-3'-like hexanucleotides separated by a three-nucleotide spacer from a similar, but less conserved hexanucleotide. Here, we report the structures of the human AR DNA binding domain (DBD) bound to two natural AREs (C3 and MTV) in head-to-head dimer conformations, diffracting at 2.05Å and 2.25Å, respectively. These structures help to explain the impact of androgen insensitivity mutations on the structure integrity, DNA binding and DBD dimerization...
April 9, 2024: Journal of Steroid Biochemistry and Molecular Biology
Lina Schiffer, Wiebke Arlt, Karl-Heinz Storbeck
Epitestosterone is a stereoisomer of the active androgen testosterone and its circulating concentrations are similar to those of testosterone in women and children. However, its biological function and pathways of metabolism remain unknown. The structural similarity to testosterone suggests a potential function in the modulation of androgen receptor signalling. It is well established that the conversion of testosterone to 5α-dihydrotestosterone enhances local androgen receptor signalling. In this study, we show that epitestosterone is metabolized to 5α-dihydroepitestosterone by both human steroid 5α-reductase isoforms, SRD5A1 and SRD5A2...
April 4, 2024: Journal of Steroid Biochemistry and Molecular Biology
Conor McGuinness, Kara L Britt
Breast cancer (BCa) is the most common cancer in women and the estrogen receptor (ER)+ subtype is increasing in incidence. There are numerous therapy options available for patients that target the ER, however issues such as innate and acquired treatment resistance, and treatment related side effects justify research into alternative therapeutic options for these patients. Patients of many solid tumour types have benefitted from immunotherapy, however response rates have been generally low in ER+ BCa. We summarise the recent work assessing CDK4/6 inhibitors for ER+ BCa and how they have been shown to prime anti-tumour immune cells and achieve impressive results in preclinical models...
March 29, 2024: Journal of Steroid Biochemistry and Molecular Biology
Lucia Cioffi, Daniela Grassi, Silvia Diviccaro, Donatella Caruso, Daniel Pinto-Benito, Maria-Angeles Arevalo, Luis Miguel Garcia-Segura, Roberto Cosimo Melcangi, Silvia Giatti
An important aspect of the neuromodulatory and neuroprotective actions exerted by neuroactive steroids is that they are sex-specific, as determined by the sexually dimorphic levels of these molecules in plasma and the nervous tissue. Thus, the identification of the factors that generate the sex-dimorphic levels of neuroactive steroids may be crucial from a neuroprotectant perspective. The main driver for sex determination in mammals is the SRY gene and the subsequent presence of a specific gonad: testes for males and ovaries for females, thus producing hormonal compounds, primarily androgens and estrogens, respectively...
March 28, 2024: Journal of Steroid Biochemistry and Molecular Biology
Andrés Elia, Gabriela Pataccini, Leo Saldain, Luisa Ambrosio, Claudia Lanari, Paola Rojas
The development of antiprogestins was initially a gynecological purpose. However, since mifepristone was developed, its application for breast cancer treatment was immediately proposed. Later, new compounds with lower antiglucocorticoid and antiandrogenic effects were developed to be applied to different pathologies, including breast cancer. We describe herein the studies performed in the breast cancer field with special focus on those reported in recent years, ranging from preclinical biological models to those carried out in patients...
March 28, 2024: Journal of Steroid Biochemistry and Molecular Biology
Agnieszka M Wojtkiewicz, Gabriela Oleksy, Magdalena A Malinowska, Tomasz Janeczko
In this study, we applied AcmB2, sourced from Sterolibacterium denitrificans, to catalyze the oxidative dehydrogenation of 3-ketolupeol (lupenone), a derivative of lupeol, triterpene obtained from birch bark. This enzymatic Δ1 -dehydrogenation catalyzed by AcmB2 yielded glochidone, a bioactive compound frequently obtained from medicinal plants like Salvia trichoclada and Maytenus boria. Glochidone is known for its broad biological activities, including antibacterial, antifungal, anti-inflammatory, anticancer, antidiabetic as well as acetylcholinesterase inhibition...
March 21, 2024: Journal of Steroid Biochemistry and Molecular Biology
Georgina Tiraboschi, Paula Isaac, María Laura Breser, Virginia Angiolini, Lucía Rodriguez-Berdini, Carina Porporatto, Luciana Paola Bohl
Mastitis is one the most widespread and serious diseases in dairy cattle. Recurrent and chronic infections are often attributable to certain pathogenicity mechanisms in mastitis-causing pathogens such as Staphylococcus spp. These include growing in biofilm and invading cells, both of which make it possible to resist or evade antimicrobial therapies and the host's immune system. This study tested the effects of active vitamin D3 (i.e., calcitriol or 1,25-dihydroxyvitamin D3 ) on the internalization and phagocytosis of biofilm-forming Staphylococcus spp...
March 21, 2024: Journal of Steroid Biochemistry and Molecular Biology
Touraj Zamir Nasta, Mohammad Reza Tabandeh, Komail Amini, Ardeshir Abbasi, Dian Dayer, Cyrus Jalili
Hyperglycemia is known as one of the main causes of infertility in human societies. Indole propionic acid (IPA) is produced by intestinal microbiota and has antioxidant and anti-inflammatory properties. This study aims to investigate the effects of IPA on molecular indices of steroidogenesis, ER stress, and apoptosis induced by high glucose (HG) in granulosa cells. Primary GCs, isolated from ovarian follicles of Rats were cultured in 5mM (control) and 30mM (HG) of glucose and in the presence of 10 and 20µM of IPA for 24h...
March 18, 2024: Journal of Steroid Biochemistry and Molecular Biology
Chaochao Gong, Sailing Chen, Yunbing Tang, Huiqian Chen, Jianghuan Xie, Yanning Lv, Zhefan Shen, Yang Zhu, Shaowei Wang, Ren-Shan Ge, Junzhao Zhao
The objective of this study was to examine the effect of 11 organochlorine pesticides on human and rat 17β-Hydroxysteroid dehydrogenase 1 (17β-HSD1) in human placental and rat ovarian microsome and on estradiol production in BeWo cells. The results showed that the IC50 values for endosulfan, fenhexamid, chlordecone, and rhothane on human 17β-HSD1 were 21.37, 73.25, 92.80, and 117.69μM. Kinetic analysis revealed that endosulfan acts as a competitive inhibitor, fenhexamid as a mixed/competitive inhibitor, chlordecone and rhothane as a mixed/uncompetitive inhibitor...
March 18, 2024: Journal of Steroid Biochemistry and Molecular Biology
Zhen-Ru Zhou, Fen Liu, Shan Li, Chang-Zhi Dong, Lei Zhang
Cytochrome P450 enzyme with 7β-hydroxylation capacity has attracted widespread attention due to the vital roles in the biosynthesis of ursodeoxycholic acid (UDCA), a naturally active molecule for the treatment of liver and gallbladder diseases. In this study, a novel P450 hydroxylase (P450FE ) was screen out from Fusarium equiseti HG18 and identified by a combination of genome and transcriptome sequencing, as well as heterologous expression in Pichia pastoris. The biotransformation of lithocholic acid (LCA) by whole cells of recombinant Pichia pastoris further confirmed the C7β-hydroxylation with 5...
March 18, 2024: Journal of Steroid Biochemistry and Molecular Biology
Despoina Kermpatsou, Frida Olsson, Erik Wåhlén, Ola Söderberg, Johan Lennartsson, Maria Norlin
The active form of vitamin D, 1,25-dihydroxyvitamin D3 , is known to act via VDR (vitamin D receptor), affecting several physiological processes. In addition, PDIA3 (protein disulphide-isomerase A3) has been associated with some of the functions of 1,25-dihydroxyvitamin D3 . In the present study we used siRNA-mediated silencing of PDIA3 in osteosarcoma and prostate carcinoma cell lines to examine the role(s) of PDIA3 for 1,25-dihydroxyvitamin D3 -dependent responses. PDIA3 silencing affected VDR target genes and significantly altered the 1,25-dihydroxyvitamin D3 -dependent induction of CYP24A1, essential for elimination of excess 1,25-dihydroxyvitamin D3 ...
March 7, 2024: Journal of Steroid Biochemistry and Molecular Biology
Reena Desai, Sasha Savkovic, David J Handelsman
Capillary dried blood spot (DBS) analysis coupled with multi-analyte steroid liquid chromatography mass spectrometry (LCMS) is attractive for field studies, home-based self-sampling as well as clinical trials by eliminating costly and laborious sample processing involving venipuncture and frozen storage/shipping while providing multiple steroid measurements from a single small sample. We investigated steroid measurements in DBS samples stored for four years at room temperature prior to analysis compared with the original venipuncture serum samples...
March 4, 2024: Journal of Steroid Biochemistry and Molecular Biology
Qinhua Gan, Haifeng Zheng, Xinyu Li, Jing Li, Jingxue Ma, Yuji Zhang, Jiakun Han, Lin Zhang, Wenxu Zhou, Yandu Lu
Phytosterols are vital structural and regulatory components in plants. Zea mays produces a series of phytosterols that are specific to corn. However, the underline biosynthetic mechanism remains elusive. In this study, we identified a novel sterol methyltransferase from Z. mays (ZmSMT1-2) which showed a unique feature compared with documented plant SMTs. ZmSMT1-2 showed a substrate preference for cycloartenol. Using S-adenosyl-L-methionine (AdoMet) as a donor, ZmSMT1-2 converted cycloartenol into alkylated sterols with unique side-chain architectures, including Δ25(27) (i...
March 4, 2024: Journal of Steroid Biochemistry and Molecular Biology
Shuopo Fang, Xiaodan Huang, Fen Cai, Guodong Qiu, Fei Lin, Xiaorui Cai
Inflammation, an important biological protective response to tissue damage or microbial invasion, is considered to be an alarming signal for the progress of varied biological complications. Based on the previous reports in the literature that proved the noticeable efficacy of pyrazole and thiazole scaffold as well as nitrogen heterocyclic based compounds against acute and chronic inflammatory disease, a new set of novel D-ring substituted steroidal 4,5-dihydropyrazole thiazole derivatives were synthesized and evaluated their anti-inflammatory activities in vitro...
February 29, 2024: Journal of Steroid Biochemistry and Molecular Biology
Marc Poirot, Luigi Iuliano, William J Griffiths, Gerard Lizard
No abstract text is available yet for this article.
February 27, 2024: Journal of Steroid Biochemistry and Molecular Biology
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