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World Journal of Microbiology & Biotechnology

Bettina Bóka, László Manczinger, Sándor Kocsubé, Kadaikunnan Shine, Naiyf S Alharbi, Jamal M Khaled, Martin Münsterkötter, Csaba Vágvölgyi, László Kredics
Several Bacillus strains are used as biocontrol agents, as they frequently have strong antagonistic effects against microbial plant pathogens. Bacillus strain SZMC 6179J, isolated from tomato rhizosphere, was previously shown to have excellent in vitro antagonistic properties against the most important fungal pathogens of tomato (Alternaria solani, Botrytis cinerea, Phytophthora infestans and Sclerotinia sclerotiorum) as well as several Fusarium species. Taxonomic investigations revealed that it is a member of the B...
March 13, 2019: World Journal of Microbiology & Biotechnology
Kaur Simranjit, Amrita Kanchan, Radha Prasanna, Kunal Ranjan, Balasubramanian Ramakrishnan, Awani Kumar Singh, Yashbir Singh Shivay
Protected cultivation of vegetables is often hampered by declining nutrient availability in soil due to year-around farming, which in turn, leads to poor quality and yields, causing serious concern. Our study aimed towards evaluating the potential of novel biofilm formulations-Anabaena or Trichoderma as matrices with Azotobacter sp. as Anabaena-Azotobacter (An-Az) and Trichoderma-Azotobacter (Tr-Az) or together as Anabaena-Trichoderma (An-Tr), on the growth, physiological activities, yield, and changes in the profiles of soil microbial communities in two cultivars (cv...
March 9, 2019: World Journal of Microbiology & Biotechnology
Tatiana Thomas, Anne Elain, Alexis Bazire, Stéphane Bruzaud
A halophilic Gram-negative eubacterium was isolated from the Iroise Sea and identified as an efficient producer of polyhydroxyalkanoates (PHA). The strain, designated SF2003, was found to belong to the Halomonas genus on the basis of 16S rRNA gene sequence similarity. Previous biochemical tests indicated that the Halomonas sp. strain SF2003 is capable of supporting various culture conditions which sometimes can be constraining for marine strains. This versatility could be of great interest for biotechnological applications...
March 9, 2019: World Journal of Microbiology & Biotechnology
Mansa Fredua-Agyeman, Simon Gaisford
Cell free supernatants (CFS) obtained from probiotic species are routinely used to preliminary investigate the antimicrobial activity of potential probiotic isolates by the agar well diffusion and broth culture. Both methods have documented limitations. In this work, the potential of isothermal microcalorimetry (IMC), a technique based on the measurement of heat produced by growing bacteria was used to investigate the antimicrobial effects of two commercial probiotic species (Lactobacillus acidophilus LA-5® and Bifidobacterium lactis BB-12® ) and one reference strain (Bifidobacterium bifidum ATCC 11863) against Pseudomonas aeruginosa NCIMB 8628 using unmodified, neutralised and heat-treated CFS...
March 6, 2019: World Journal of Microbiology & Biotechnology
Bastian Schorr, Hanadi Ghanem, Stefan Rosiwal, Walter Geißdörfer, Andreas Burkovski
Boron-doped diamond electrodes can be used to generate reactive oxygen species directly at the electrode's surface. This property was used in this study for in-situ electrochemical oxidation to eliminate different bacteria, i.e. Escherichia coli, Pseudomonas fluorescens and Pseudomonas aeruginosa, as well as Bacillus subtilis spores from water samples. Application of low voltages in the rage from 4 to 10 V and short incubation times in the range of minutes allowed a complete disinfection of water contaminated with enterobacteria and freshwater microbes including nosocomial pathogens as well as a significant reduction of spores...
March 6, 2019: World Journal of Microbiology & Biotechnology
K C Kumawat, Poonam Sharma, Asmita Sirari, Inderjeet Singh, B S Gill, Ummed Singh, Krishna Saharan
The present study was aimed to assess the scope of native potential endophyte Pseudomonas aeruginosa (LSE-2) strain (KX925973) with recommended Bradyrhizobium sp. (LSBR-3) (KF906140) for synergistic effect to develop as consortium biofertilizer of soybean. A total of 28 non-rhizobial endophytic bacteria were isolated from cultivated and wild sp. of soybean. All isolates were screened for multifarious PGP traits viz. Indole-3-acetic acid (IAA), phosphate (P) and zinc (Zn) solubilization, siderophore, cell wall degrading enzymes and pathogenicity...
March 5, 2019: World Journal of Microbiology & Biotechnology
Zhongkang Wang, Jie Yang, Caiyan Xin, Xiaorui Xing, Youping Yin, Li Chen, Zhangyong Song
Microsclerotia (MS) produced in the liquid culture of the dimorphic insect pathogen Metarhizium rileyi can be used as a mycoinsecticide. Bioinformatics analysis demonstrated that the cell cycle signaling pathway was involved in regulating MS formation. To investigate the mechanisms by which the signaling pathway is regulated, a cell cycle box binding transcription factor MrSwi6 of M. rileyi was characterized. MrSwi6 was highly expressed during periods of yeast-hypha transition and conidia and MS formation. When compared with wild-type and complemented strains, disruption of MrSwi6 significantly reduced conidia (15-36%) and MS formation (96...
March 1, 2019: World Journal of Microbiology & Biotechnology
Saša Simčič, Aleš Berlec, Sanja Stopinšek, Borut Štrukelj, Rok Orel
Dysbiosis of intestinal microbiota and aberrant inflammatory responses in gastrointestinal mucosa plays important roles in the development of inflammatory bowel disease (IBD). The purpose of this study was to demonstrate the probiotic activity of Lactococcus lactis and the ability of TNF-α-binding by recombinant L. lactis bearing TNF-α-binding affibodies. Various concentrations of recombinant L. lactis were exposed to TNF-α and its binding measured by ELISA. Mucosal biopsies of patients with active IBD were incubated with various L...
February 27, 2019: World Journal of Microbiology & Biotechnology
Mohamed Maizatul-Suriza, Matthew Dickinson, Abu Seman Idris
Bud rot disease is a damaging disease of oil palm in Colombia. The pathogen responsible for this disease is a species of oomyctes, Phytophthora palmivora which is also the causal pathogen of several tropical crop diseases such as fruit rot and stem canker of cocoa, rubber, durian and jackfruit. No outbreaks of bud rot have been reported in oil palm in Malaysia or other Southeast Asian countries, despite this particular species being present in the region. Analysis of the genomic sequences of several genetic markers; the internal transcribe spacer regions (ITS) of the ribosomal RNA gene cluster, beta-tubulin gene, translation elongation factor 1 alpha gene (EF-1α), cytochrome c oxidase subunit I & II (COXI and COXII) gene cluster along with amplified fragment length polymorphism (AFLP) analyses have been carried out to investigate the genetic diversity and variation of P...
February 27, 2019: World Journal of Microbiology & Biotechnology
Qingfeng Cheng, Yang Huang, Lichao Nengzi, Jianying Liu, Jie Zhang
Dissolved oxygen (DO) is a significant operational parameter in biological systems. In this study, a pilot-scale biofilter was constructed to investigate the removal efficiency of ammonia, iron and manganese, as well as the microbial community structure evolution at different DO concentrations. Results indicated that when DO decreased from 8 to 4 mg/L, iron and manganese were still completely removed, however the concentration of ammonia in the effluent increased, and exceeded the permitted limit of 0.5 mg/L when DO was about 4 mg/L...
February 19, 2019: World Journal of Microbiology & Biotechnology
Anhui Gong, Tao Zhou, Chenghong Xiao, Weike Jiang, Yongqiang Zhou, Jinqiang Zhang, Qing Liang, Changgui Yang, Wei Zheng, Chenggang Zhang
Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides have not been analyzed in detail. In this study, we isolated and identified 46 endophytic fungal strains from the taproots, lateral roots and leaves, and we used morphological and molecular biological methods to assign them into 15 genera: Fusarium sp...
February 18, 2019: World Journal of Microbiology & Biotechnology
Glêzia Renata da Silva Lacerda, Jeanne Cristina Lapenda Lins Cantalice, Gláucia Manoella de Souza Lima, Luiz Eduardo Félix de Albuquerque, Isllan D'Erik Gonçalves da Silva, Maria Eliane Bezerra de Melo, Mônica Lúcia Adam, Silene Carneiro do Nascimento
L-asparaginase is an enzyme capable of hydrolyzing the substrate asparagine in aspartic acid and ammonia. Due to this mechanism of action observed, L-asparaginase is widely used in the treatment of Acute Lymphoblastic Leukemia, since these cells use asparagine for their survival. Because it is frequently used as an antineoplastic, it is necessary to evaluate its genotoxic effects. The aim of the present study was to evaluate cellular DNA damage after exposure to L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420...
February 14, 2019: World Journal of Microbiology & Biotechnology
Hadi Eslami, Ahmad Shariatifar, Ebrahim Rafiee, Mahboubeh Shiranian, Faezeh Salehi, Saeede Sadat Hosseini, Gilda Eslami, Reza Ghanbari, Ali Asghar Ebrahimi
The present study investigated biodegradation and removal of Reactive Red 198 (RR198) dye from aqueous environments using a new bacterial consortium isolated from textile wastewater sludge on laboratory scale via batch study. Two bacterial species, Enterococcus faecalis (EF) and Klebsiella variicola (KV), were identified after isolation, through biochemical assays, Polymerase chain reaction (PCR), and 16S rRNA gene sequencing. To determine their ability to biodegrade RR198 dye, physicochemical parameters, including bacterial concentration, time, pH, and temperature, were tested; the results showed that the best conditions included a bacterial concentration of 3...
February 9, 2019: World Journal of Microbiology & Biotechnology
João Pedro Rueda Furlan, André Pitondo-Silva, Vânia Santos Braz, Inara Fernanda Lage Gallo, Eliana Guedes Stehling
The correct identification of different genera and bacterial species is essential, especially when these bacteria cause infections and appropriate therapies need to be chosen. Bacteria belonging to the Burkholderia cepacia complex are considered important opportunistic pathogens, causing different types of infections in immunocompromised, principally in patients with cystic fibrosis. Twenty-one isolates were obtained from different soil samples and identified by sequencing of 16S rRNA, 23S rRNA, recA gene, MLST and by VITEK 2 and MALDI-TOF MS systems...
February 9, 2019: World Journal of Microbiology & Biotechnology
Qassim Esmaeel, Cédric Jacquard, Christophe Clément, Lisa Sanchez, Essaid Ait Barka
Grey mould caused by Botrytis cinerea is among the most important disease affecting the production of grapevine worldwide. The high economical loss each year has led producers to become more dependent on chemical pesticides for protection. However, environmental impacts of the pesticides overuse have sparked crescent interest in developing alternative biocontrol methods. The use of plant-associated bacteria has, thus, received many attentions as a promising strategy for sustainable agriculture. Three strains, isolated from the rhizosphere of crops cultivated in the northeast of France, were evaluated for their antagonistic effect...
February 9, 2019: World Journal of Microbiology & Biotechnology
Ryosuke Yamada, Koichi Ogura, Yusuke Kimoto, Hiroyasu Ogino
With the reduction in oil reserves and steady increases in the price of oil, alternative carbon sources like methanol are promising, but an efficient conversion process to fuels and other chemicals is still desired. In this study, we demonstrated for the first time the production of lactic acid from methanol using a lactate dehydrogenase copy number amplifying strategy in Pichia pastoris. We engineered methylotrophic yeast (Pichia pastoris) producing D-lactic acid by D-lactate dehydrogenase gene (d-LDH) integration into the non-transcribed spacer of the ribosomal DNA (rDNA) locus and post-transformational amplification...
February 4, 2019: World Journal of Microbiology & Biotechnology
Costanza Baldisserotto, Alessandra Sabia, Lorenzo Ferroni, Simonetta Pancaldi
As major primary producers in marine environments, diatoms are considered a valuable feedstock of biologically active compounds for application in several biotechnological fields. Due to their metabolic plasticity, especially for light perception and use and in order to make microalgal production more environmentally sustainable, marine diatoms are considered good candidates for the large-scale cultivation. Among physical parameters, light plays a primary role. Even if sunlight is cost-effective, the employment of artificial light becomes a winning strategy if a high-value microalgal biomass is produced...
February 2, 2019: World Journal of Microbiology & Biotechnology
Guangzheng Sun, Chanjing Feng, Ancheng Zhang, Yishuai Zhang, Dongwei Chang, Yang Wang, Qing Ma
In order to define the role of oxalic acid (OA) in the invasion of Botrytis cinerea in tomato plants, the OA induction of resistance related to oxalate oxidase (O×O) and germin was examined. In greenhouse experiments, OA at 3 mmol/L significantly induced resistance in tomato plants against B. cinerea strains B05.10 and T4, reducing lesion size of 37.55% and 24.91% by compared with distilled water control, respectively, while 20 mmol/L OA increasing by 36.14% and 41.48%. OA contents were 98 and 46 µg/mL when tomato plants were infected by B...
February 2, 2019: World Journal of Microbiology & Biotechnology
Arabinda Ghosh, Saikat Sutradhar, Debabrat Baishya
In this present study novel endoxylanase producing Bacillus licheniformis DM5 isolated, identified based on 16S rDNA from Garampani hotspring, Assam, India and enzyme was purified. RNA secondary structure predicted the similarity of B. licheniformis DM5 with B. licheniformis ATCC14580. Highest production of xylanase from B. licheniformis DM5 was achieved in the TY medium with cell densities 12 g/l and extracellular protein concentration containing xylanase 400 mg/l. Partially purified extracellular xylanase displayed optimum pH 6...
January 31, 2019: World Journal of Microbiology & Biotechnology
Ning Xu, Liang Wei, Jun Liu
Cell metabolism in living organisms is largely regulated at the transcriptional level, and the promoters are regarded as basic regulatory elements responsible for transcription initiation. Promoter engineering is an important technique to regulate gene expression and optimize metabolite biosynthesis in metabolic engineering and synthetic biology. The rational and precise control of gene expression in the multi-gene pathways can significantly affect the metabolic flux distribution and maximize the production of specific metabolites...
January 31, 2019: World Journal of Microbiology & Biotechnology
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