Seiwa Horie, Chihiro Nakatomi, Misa Ito-Sago, Aoi Morii, Ai Orimoto, Hiroshi Ikeda, Chia-Chien Hsu, Mako Naniwa, Masahiro Mizuhara, Kaori Gunjigake, Tatsuo Kawamoto, Kentaro Ono
OBJECTIVES: Orthodontic mechanical force on the periodontal ligament induces extracellular adenosine triphosphate (ATP) release. However, mechanosensitive molecules have not been confirmed functionally in periodontal ligament cells. In the present study, we examined the roles of mechanosensitive PIEZO channels in the mechanically stimulated release of ATP in human periodontal ligament fibroblasts (HPdLFs). MATERIALS AND METHODS: To examine PIEZO expression in HPdLFs, we performed reverse transcription-quantitative polymerase chain reaction, fluorescent immunostaining, and Ca2+ imaging...
August 26, 2023: European Journal of Orthodontics