journal
https://read.qxmd.com/read/38907695/dynamic-observation-of-the-membrane-interaction-processes-of-%C3%AE-lactoglobulin-by-time-resolved-vacuum-ultraviolet-circular-dichroism
#1
JOURNAL ARTICLE
Satoshi Hashimoto, Koichi Matsuo
The elucidation of protein-membrane interactions is pivotal for comprehending the mechanisms underlying diverse biological phenomena and membrane-related diseases. In this investigation, vacuum-ultraviolet circular dichroism (VUVCD) spectroscopy, utilizing synchrotron radiation (SR), was employed to dynamically observe membrane interaction processes involving water-soluble proteins at the secondary-structure level. The study utilized a time-resolved (TR) T-shaped microfluidic cell, facilitating the rapid and efficient mixing of protein and membrane solutions...
June 22, 2024: Analytical Chemistry
https://read.qxmd.com/read/38905705/quartz-crystal-microbalance-frequency-response-to-discrete-adsorbates-in-liquids
#2
JOURNAL ARTICLE
Alexander M Leshansky, Boris Y Rubinstein, Itzhak Fouxon, Diethelm Johannsmann, Marta Sadowska, Zbigniew Adamczyk
Quartz crystal microbalance with dissipation monitoring (QCM-D) has become a major tool enabling accurate investigation of the adsorption kinetics of nanometric objects such as DNA fragments, polypeptides, proteins, viruses, liposomes, polymer, and metal nanoparticles. However, in liquids, a quantitative analysis of the experimental results is often intricate because of the complex interplay of hydrodynamic and adhesion forces varying with the physicochemical properties of adsorbates and functionalized QCM-D sensors...
June 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38904303/nanoscale-quantitative-imaging-of-single-nuclear-pore-complexes-by-scanning-electrochemical-microscopy
#3
JOURNAL ARTICLE
Ran Chen, Pavithra Pathirathna, Ryan J Balla, Jiyeon Kim, Shigeru Amemiya
The nuclear pore complex (NPC) is a proteinaceous nanopore that solely and selectively regulates the molecular transport between the cytoplasm and nucleus of a eukaryotic cell. The ∼50 nm-diameter pore of the NPC perforates the double-membrane nuclear envelope to mediate both passive and facilitated molecular transport, thereby playing paramount biological and biomedical roles. Herein, we visualize single NPCs by scanning electrochemical microscopy (SECM). The high spatial resolution is accomplished by employing ∼25 nm-diameter ion-selective nanopipets to monitor the passive transport of tetrabutylammonium at individual NPCs...
June 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38904276/programmable-dna-nanomachine-integrated-with-electrochemically-controlled-atom-transfer-radical-polymerization-for-antibody-detection-at-picomolar-level
#4
JOURNAL ARTICLE
Baoting Dou, Keming Wang, Yan Chen, Po Wang
The quantitative detection of antibodies is crucial for the diagnosis of infectious and autoimmune diseases, while the traditional methods experience high background signal noise and restricted signal gain. In this work, we have developed a highly efficient electrochemical biosensor by constructing a programmable DNA nanomachine integrated with electrochemically controlled atom transfer radical polymerization (eATRP). The sensor works by binding the target antidigoxin antibody (anti-Dig) to the epitope of the recognization probe, which then initiates the cascaded strand displacement reaction on a magnetic bead, leading to the capture of cupric oxide (CuO) nanoparticles through magnetic separation...
June 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38904260/multilayer-annotation-strategy-annoseps-disentangling-the-intricate-structure-of-selenium-containing-polysaccharides-based-on-preferential-fragmentation-patterns
#5
JOURNAL ARTICLE
Xixuan Wu, Wei Jia
Precision mapping of selenium at structural and position levels poses significant challenges in selenium-containing polysaccharide identification. Due to the absence of reference spectra, database-centric approaches are still limited in the discovery of selenium binding sites and distinction among different isomeric structures. A multilayer annotation strategy, AnnoSePS, is proposed for achieving the identification of seleno-substituent and the unbiased profiling of polysaccharides. Applying Snoop-triggered multiple reaction monitoring (Snoop-MRM) identified multidimensional monosaccharides in selenium-containing polysaccharides...
June 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38904228/ultrasensitive-and-wash-free-detection-of-tumor-extracellular-vesicles-by-aptamer-proximity-ligation-activated-rolling-circle-amplification-coupled-to-single-particle-icp-ms
#6
JOURNAL ARTICLE
Xue-Wei Zhang, Gong-Xiang Qi, Shuai Chen, Yong-Liang Yu, Jian-Hua Wang
Tumor-derived extracellular vesicles (TEVs) are rich in cellular information and hold great promise as a biomarker for noninvasive cancer diagnosis. However, accurate measurement of TEVs presents challenges due to their low abundance and potential interference from a high number of EVs derived from normal cells. Herein, an aptamer-proximity-ligation-activated rolling circle amplification (RCA) method for EV membrane recognition, coupled with single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) for the quantification of TEVs, is developed...
June 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38902946/monitoring-poly-methyl-methacrylate-and-polyvinyl-dichloride-micro-nanoplastics-in-water-by-direct-solid-phase-microextraction-coupled-to-gas-chromatography-mass-spectrometry
#7
JOURNAL ARTICLE
Shengrui Xu, Huimin Li, Li Xiao, Suling Feng, Jing Fan, Janusz Pawliszyn
A simple, sustainable, and sensitive monitoring approach of micro/nanoplastics (MNPs) in aqueous samples is crucial since it helps in assessing the extent of contamination and understanding the potential risks associated with their presence without causing additional stress to the environment. In this study, a novel strategy for qualitative and quantitative determination of MNPs in water by direct solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was proposed for the first time...
June 20, 2024: Analytical Chemistry
https://read.qxmd.com/read/38901019/mitochondria-targetable-near-infrared-fluorescent-probe-for-visualization-of-hydrogen-peroxide-in-lung-injury-liver-injury-and-tumor-models
#8
JOURNAL ARTICLE
Qi Zan, Kunyi Zhao, Ruijin Li, Yongming Yang, Xihua Yang, Wenzhong Li, Gangli Zhang, Chuan Dong, Shaomin Shuang, Li Fan
Hydrogen peroxide (H2 O2 ) overexpressed in mitochondria has been regarded as a key biomarker in the pathological processes of various diseases. However, there is currently a lack of suitable mitochondria-targetable near-infrared (NIR) probes for the visualization of H2 O2 in multiple diseases, such as PM2.5 exposure-induced lung injury, hepatic ischemia-reperfusion injury (HIRI), nonalcoholic fatty liver (NAFL), hepatic fibrosis (HF), and malignant tumor tissues containing clinical cancer patient samples. Herein, we conceived a novel NIR fluorescent probe ( HCy-H 2 O 2 ) by introducing pentafluorobenzenesulfonyl as a H2 O2 sensing unit into the NIR hemicyanine platform...
June 20, 2024: Analytical Chemistry
https://read.qxmd.com/read/38898770/cspbbr-3-perovskite-quantum-dots-encapsulated-by-a-polymer-matrix-for-ultrasensitive-dynamic-imaging-of-intracellular-microrna
#9
JOURNAL ARTICLE
Zezhou Yang, Jie Zhou, Fang Liu, Yaqin Chai, Pu Zhang, Ruo Yuan
Herein, CsPbBr3 perovskite quantum dots (CPB PQDs)@poly(methyl methacrylate) (PMMA) (CPB@PMMA) nanospheres were used as energy donors with high Förster resonance energy transfer (FRET) efficiency and exceptional biocompatibility for ultrasensitive dynamic imaging of tiny amounts of microRNAs in living cells. Impressively, compared with traditional homogeneous single QDs as energy donors, CPB@PMMA obtained by encapsulating numerous CPB PQDs into PMMA as energy donors could not only significantly increase the efficiency of FRET via improving the local concentration of CPB PQDs but also distinctly avoid the problem of cytotoxicity caused by divulged heavy metal ions entering living cells...
June 19, 2024: Analytical Chemistry
https://read.qxmd.com/read/38896549/semiarbitrary-qpcr-for-sensitive-detection-of-circulating-mirna-via-terminal-deoxynucleotidyl-transferase-assisted-rna-primed-dna-polymerization
#10
JOURNAL ARTICLE
Jingyu Cui, Jiafang Piao, Houyu Han, Weipan Peng, Mengyao Lin, Dianming Zhou, Cheng Zhu, Xiaoqun Gong
Circulating microRNAs (miRNAs) have recently emerged as noninvasive disease biomarkers. Quantitative detection of circulating miRNAs could offer significant information for clinical diagnosis due to its significance in the development of biological processes. In response to the current challenges of circulating miRNA detection, we introduce a sensitive, selective, and versatile circulating miRNA detection strategy using terminal deoxynucleotidyl transferase (TdT)-catalyzed RNA-primed DNA polymerization (TCRDP) coupled with semiarbitrary qPCR (SAPCR)...
June 19, 2024: Analytical Chemistry
https://read.qxmd.com/read/38896456/capture-and-detection-of-aerosolized-fentanyl-in-a-suspended-electrochemical-cell
#11
JOURNAL ARTICLE
Kathryn J Vannoy, Lynn E Krushinski, Jeffrey E Dick
Fentanyl is an extremely potent opioid that is commonly laced into other drugs. Fentanyl poses a danger to users but also to responders or bystanders who may unknowingly ingest a lethal dose (∼2 mg) of fentanyl from aerosolized powder or vapor. Electrochemistry offers a small, simple, and affordable platform for the direct detection of illicit substances; however, it is largely limited to solution-phase measurements. Here, we demonstrate the hands-free capture and electroanalyzation of aerosols containing fentanyl...
June 19, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889444/identification-of-the-binding-site-between-aptamer-sgc8c-and-ptk7
#12
JOURNAL ARTICLE
Jianghuai Chen, Jiaxuan He, Tao Bing, Yawei Feng, Yifan Lyu, Ming Lei, Weihong Tan
Aptamers are single-stranded RNA or DNA molecules that can specifically bind to targets and have found broad applications in cancer early-stage detection, accurate drug delivery, and precise treatment. Although various aptamer screening methods have been developed over the past several decades, the accurate binding site between the target and the aptamer cannot be characterized during a typical aptamer screening process. In this research, we chose a widely used aptamer screened by our group, sgc8c, and its target protein tyrosine kinase 7 (PTK7) as the model aptamer and target and tried to determine the binding site between aptamer sgc8c and PTK7...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889311/stimulus-responsive-four-stranded-dna-nanoring-assembly-to-host-multiple-nanosilver-clusters-for-cooperatively-enhanced-fluorescence-biosensing
#13
JOURNAL ARTICLE
Xinyue Jia, Jiayang He, Mengdie Li, Jingjing Ye, Yuqing Zhang, Chunli Yang, Ruo Yuan, Wenju Xu
Exploring the ability of four-stranded DNA nanorings ( fs DNRs) to host multiple nanosilver clusters (NAgCs) for cooperatively amplifiable fluorescence biosensing to a specific initiator ( t I* ) is fascinating. By designing three DNA single strands and three analogous stem-loop hairpins, we developed a functional fs DNR through sequential cross-opening and overlapped hybridization. Note that a substrate strand (SS) was programmed with six modules: two severed splits (sT and sT') of NAgCs template, two sequestered segments by a middle unpaired spacer, and a partition for t I* -recognizable displacement, while sT and sT' were also tethered in two ends of three hairpins...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889297/multichannel-nanogenerator-driven-collaborative-metabolic-fingerprint-diagnostic-strategy-for-early-screening-and-risk-evaluation-of-nonalcoholic-fatty-liver-disease
#14
JOURNAL ARTICLE
Chenjie Yang, Da Zhou, Hailong Yu, Yijie Chen, Hairu Lin, Hao Wu, Chunhui Deng
Nonalcoholic fatty liver disease (NAFLD), along with its progressive forms nonalcoholic steatohepatitis (NASH) and NASH fibrosis, has emerged as a global health crisis. However, the absence of robust screening and risk evaluation tools contributes to the underdiagnosis of NAFLD. Herein, we reported a multichannel nanogenerator-assisted laser desorption/ionization mass spectrometry (LDI-MS) platform for early screening and risk evaluation of NAFLD. Specifically, titanium oxide nanosheets (TiNS) and covalent-organic framework nanosheets (COFNS) were employed as nanogenerators with excellent optical properties and exhibited efficient desorption/ionization during the LDI-MS process...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889191/label-free-screening-of-drug-induced-liver-injury-using-stimulated-raman-scattering-microscopy-and-spectral-phasor-analysis
#15
JOURNAL ARTICLE
William J Tipping, Liam T Wilson, Nicholas C O Tomkinson, Karen Faulds, Duncan Graham
Hepatic toxicity is a leading cause of the termination of clinical trials and the withdrawal of therapeutics following regulatory approval. The detection of drug-induced liver injury (DILI) is therefore of importance to ensure patient safety and the effectiveness of novel small molecules and drugs. DILI encompasses drug-induced steatosis (DIS) and drug-induced phospholipidosis (DIPL) which involve the accumulation of excess intracellular lipids. Here, we develop hyperspectral stimulated Raman scattering (SRS) microscopy as a label-free methodology for discriminating DIS and DIPL in mammalian cell culture...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889184/near-infrared-spontaneously-blinking-fluorophores-for-live-cell-super-resolution-imaging-with-minimized-phototoxicity
#16
JOURNAL ARTICLE
Song Chen, Jing Wang, Daoming Guan, Baojin Tan, Tianli Zhai, Lu Yang, Yuheng Han, Yi Liu, Qian Liu, Yunxiang Zhang
Single-molecule localization microscopy (SMLM) requires high-intensity laser irradiation, typically exceeding kW/cm2 , to yield a sufficient photon count. However, this intense visible light exposure incurs substantial cellular toxicity, hindering its use in living cells. Here, we developed a class of near-infrared (NIR) spontaneously blinking fluorophores for SMLM. These NIR fluorophores are a combination of rhodamine spirolactams and merocyanine derivatives, where the rhodamine spirolactam component converts between a bright and dark state based on pH-dependent spirocyclization and merocyanine derivatives shift the excitation wavelength into the infrared...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889097/k-specification-with-flavone-p0-probe-in-a-g-quadruplex-dna
#17
JOURNAL ARTICLE
Wenqin Zhou, Wang Wan, Wenhui Miao, Yu Bao, Yu Liu, Guoqing Jia, Can Li
G-quadruplex (G4) DNA is considered as a prospective therapeutic target due to its potential biological significance. To understand G4 biological roles and function, a G4-specific fluorescent probe is necessary. However, it is difficult for versatile G4 to precisely recognize without perturbing their folding dynamics. Herein, we reported that flavone P0 can be a fluorescent probe for G4 DNA-specific recognition and have developed a highly selective detection of K+ ion by dimeric G4/P0 system. When comparing various nucleic acid structures, including G4, i-motif, ss/ds-DNA, and triplex, an apparent fluorescence enhancement is observed in the presence of G4 DNA for 85-fold, but only 8-fold for non-G4 DNA...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38889002/separation-of-activated-t-cells-using-multidimensional-double-spiral-mdds-inertial-microfluidics-for-high-efficiency-car-t-cell-manufacturing
#18
JOURNAL ARTICLE
Hyungkook Jeon, Caleb R Perez, Taeyoon Kyung, Michael E Birnbaum, Jongyoon Han
This study introduces a T cell enrichment process, capitalizing on the size differences between activated and unactivated T cells to facilitate the isolation of activated, transducible T cells. By employing multidimensional double spiral (MDDS) inertial sorting, our approach aims to remove unactivated or not fully activated T cells post-activation, consequently enhancing the efficiency of chimeric antigen receptor (CAR) T cell manufacturing. Our findings reveal that incorporating a simple, label-free, and continuous MDDS sorting step yields a purer T cell population, exhibiting significantly enhanced viability and CAR-transducibility (with up to 85% removal of unactivated T cells and approximately 80% recovery of activated T cells); we found approximately 2-fold increase in CAR transduction efficiency for a specific sample, escalating from ∼10% to ∼20%, but this efficiency highly depends on the original T cell sample as MDDS sorting would be more effective for samples possessing a higher proportion of unactivated T cells...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38888386/using-the-quartz-crystal-microbalance-to-monitor-the-curing-of-drying-oils
#19
JOURNAL ARTICLE
Gwen dePolo, Arnaud Lesaine, Marco Faustini, Lucie Laporte, Côme Thillaye du Boullay, Étienne Barthel, Joen Hermans, Piet D Iedema, Laurence de Viguerie, Kenneth R Shull
Drying oils such as linseed oil form a polymer network through a complex free-radical polymerization process. We have studied polymerization in this challenging class of polymers using a quartz crystal microbalance (QCM). The QCM is able to measure the evolution of polymer mass and mechanical properties as the oil transitions from a liquid-like to a solid-like state. Measurements using bulk materials and thin films provide information about the initial polymerization phase as well as the evolution of the mass and mechanical properties over the first two years of cure...
June 18, 2024: Analytical Chemistry
https://read.qxmd.com/read/38888104/a-complementary-multitechnique-approach-to-assess-the-bias-in-molecular-weight-determination-of-lignin-by-derivatization-free-gel-permeation-chromatography
#20
JOURNAL ARTICLE
Daniel Papp, Göran Carlström, Tommy Nylander, Margareta Sandahl, Charlotta Turner
The growing interest in lignin valorization in the past decades calls for analytical techniques for lignin characterization, ranging from wet chemistry techniques to highly sophisticated chromatographic and spectroscopic methods. One of the key parameters to consider is the molecular weight profile of lignin, which is routinely determined by size-exclusion chromatography; however, this is by no means straightforward and is prone to being hampered by considerable errors. Our study expands the fundamental understanding of the bias-inducing mechanisms in gel permeation chromatography (GPC), the magnitude of error originating from using polystyrene standards for mass calibration, and an evaluation of the effects of the solvent and type of lignin on the observed bias...
June 18, 2024: Analytical Chemistry
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