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Vijay M Miriyala, Sanyasi Sitha, Paul A Steenkamp, Ntakadzeni E Madala
RATIONALE: Metabolomics is a qualitative and quantitative measurement of the metabolite content of any biological system under a given physiological status. Due to the chemically diverse nature of these samples, metabolite identification is a difficult task, and development of alternative approaches, such as those based on mass spectrometry (MS), aimed at proper metabolite identification is required. METHODS: Compliance constants, a direct measure of mechanical bond strength, were used for the first time to predict the MS fragmentation patterns of different regional isomers of a ubiquitous plant metabolite, caffeoylquinic acid (CQA)...
October 30, 2015: Rapid Communications in Mass Spectrometry: RCM
Lili Li, Xin Lu, Jieyu Zhao, Junjie Zhang, Yanni Zhao, Chunxia Zhao, Guowang Xu
The combination of the lipidome and the metabolome can provide much more information in plant metabolomics studies. A method for the simultaneous extraction of the lipidome and the metabolome of fresh tobacco leaves was developed. Method validation was performed on the basis of the optimal ratio of methanol to methyl tert-butyl ether to water (37:45:68) from the design of experiments. Good repeatability was obtained. We found that 92.2% and 91.6% of the peaks for the lipidome and the metabolome were within a relative standard deviation of 20%, accounting for 94...
July 2015: Analytical and Bioanalytical Chemistry
Mariateresa Maldini, Fausta Natella, Simona Baima, Giorgio Morelli, Cristina Scaccini, James Langridge, Giuseppe Astarita
The consumption of vegetables belonging to the family Brassicaceae (e.g., broccoli and cauliflower) is linked to a reduced incidence of cancer and cardiovascular diseases. The molecular composition of such plants is strongly affected by growing conditions. Here we developed an unbiased metabolomics approach to investigate the effect of light and dark exposure on the metabolome of broccoli sprouts and we applied such an approach to provide a bird's-eye view of the overall metabolic response after light exposure...
2015: International Journal of Molecular Sciences
Rony Chamoun, Konstantinos A Aliferis, Suha Jabaji
Stachybotrys elegans is able to parasitize the fungal plant pathogen Rhizoctonia solani AG-3 following a complex and intimate interaction, which, among others, includes the production of cell wall-degrading enzymes, intracellular colonization, and expression of pathogenic process encoding genes. However, information on the metabolome level is non-existent during mycoparasitism. Here, we performed a direct-infusion mass spectrometry (DIMS) metabolomics analysis using an LTQ Orbitrap analyzer in order to detect changes in the profiles of induced secondary metabolites of both partners during this mycoparasitic interaction 4 and 5 days following its establishment...
2015: Frontiers in Microbiology
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