Bruno Paiva, Luis A Corchete, Maria-Belen Vidriales, Ramón García-Sanz, Jose J Perez, Irene Aires-Mejia, Maria-Luz Sanchez, Paloma Barcena, Diego Alignani, Cristina Jimenez, Maria-Eugenia Sarasquete, María-Victoria Mateos, Enrique M Ocio, Noemi Puig, Fernando Escalante, José Hernández, Rebeca Cuello, Alfonso García de Coca, Magdalena Sierra, Maria-Carmen Montes, Tomás J González-López, Josefina Galende, Abelardo Bárez, José Alonso, Emilia Pardal, Alberto Orfao, Norma C Gutierrez, Jesús F San Miguel
Although information about the molecular pathogenesis of Waldenström macroglobulinemia (WM) has significantly advanced, the precise cell of origin and the mechanisms behind WM transformation from immunoglobulin-M (IgM) monoclonal gammopathy of undetermined significance (MGUS) remain undetermined. Here, we undertook an integrative phenotypic, molecular, and genomic approach to study clonal B cells from newly diagnosed patients with IgM MGUS (n = 22), smoldering (n = 16), and symptomatic WM (n = 11). Through principal component analysis of multidimensional flow cytometry data, we demonstrated highly overlapping phenotypic profiles for clonal B cells from IgM MGUS, smoldering, and symptomatic WM patients...
April 9, 2015: Blood