Mohamad R Khazaei, Marie-Pier Girouard, Ricardo Alchini, Stephan Ong Tone, Tadayuki Shimada, Susanne Bechstedt, Mitra Cowan, Dominique Guillet, Paul W Wiseman, Gary Brouhard, Jean Francois Cloutier, Alyson E Fournier
Coordinated control of the growth cone cytoskeleton underlies axon extension and guidance. Members of the collapsin response mediator protein (CRMP) family of cytosolic phosphoproteins regulate the microtubule and actin cytoskeleton, but their roles in regulating growth cone dynamics remain largely unexplored. Here, we examine how CRMP4 regulates the growth cone cytoskeleton. Hippocampal neurons from CRMP4-/- mice exhibited a selective decrease in axon extension and reduced growth cone area, whereas overexpression of CRMP4 enhanced the formation and length of growth cone filopodia...
October 24, 2014: Journal of Biological Chemistry