Maurizio Bruschi, Maricla Galetti, Renato Alberto Sinico, Gabriella Moroni, Alice Bonanni, Antonella Radice, Angela Tincani, Federico Pratesi, Paola Migliorini, Corrado Murtas, Franco Franceschini, Barbara Trezzi, Francesca Brunini, Rita Gatti, Regina Tardanico, Giancarlo Barbano, Giorgio Piaggio, Piergiorgio Messa, Pietro Ravani, Francesco Scolari, Giovanni Candiano, Alberto Martini, Landino Allegri, Gian Marco Ghiggeri
Glomerular planted antigens (histones, DNA, and C1q) are potential targets of autoimmunity in lupus nephritis (LN). However, the characterization of these antigens in human glomeruli in vivo remains inconsistent. We eluted glomerular autoantibodies recognizing planted antigens from laser-microdissected renal biopsy samples of 20 patients with LN. Prevalent antibody isotypes were defined, levels were determined, and glomerular colocalization was investigated. Renal and circulating antibodies were matched, and serum levels were compared in 104 patients with LN, 84 patients with SLE without LN, and 50 patients with rheumatoid arthritis (RA)...
August 2015: Journal of the American Society of Nephrology: JASN