JOURNAL ARTICLE

Phosphorus metabolites in different muscles of the rat leg by 31P image-selected in vivo spectroscopy

B Madhu, K Lagerwall, B Soussi
NMR in Biomedicine 1996, 9 (8): 327-32
9176886
The difference in concentration of phosphorylated metabolites in muscles with different fiber composition was studied in vivo by localized 31P nuclear magnetic resonance spectroscopy in the rat hindlimb 120-160 microliters volumes were selected in regions containing the soleus and gastrocnemius muscles. Concentrations of phosphocreatine (PCr), adenosine triphosphate and inorganic phosphate (Pi) were determined and intracellular pH was calculated in the respective muscle groups. The highest level of PCr was found in the gastrocnemius muscle, containing 30.7 mmoles/dm3 tissue compared to 22.3 mmoles/dm3 in the soleus muscle. Pi was significantly lower in gastrocnemius (1.9 mmoles/dm3) than in soleus (3.2 mmoles/dm3). The ATP concentration was 6.7 and 6.4 mmoles/dm3 and pH was determined to 7.11 and 7.09 in the gastrocnemius and soleus muscle, respectively. Our NMR data show that it is possible to measure high-energy phosphates with precision in small localized volumes with the ISIS method using a Helmholtz coil. Earlier biochemical data are confirmed by these in vivo NMR results. Localized in vivo 31P NMR spectroscopy can contribute to the understanding of the underlying mechanisms of several metabolic events in different regions of the tissue. The method can be used for future studies of varying ischemia tolerance, varying degrees of adaptation to exercise with regard to oxidative capacity, and pH compartmentation in muscles with different fiber composition.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Trending Papers

Remove bar
Read by QxMD icon Read
9176886
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"