We have located links that may give you full text access.
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Differential regulation of gap junction protein (connexin) genes during cardiomyocytic differentiation of mouse embryonic stem cells in vitro.
Experimental Cell Research 1996 December 16
Using an in vitro system for differentiation of embryonic stem (ES) cells into cardiac myocytes, we analyzed the expression of connexin (Cx) genes by RT-PCR to learn what changes in the expression of multiple connexin genes occur during the early stage of heart development. We also examined gap junctional intercellular communication by using Lucifer Yellow dye microinjection transfer, studied intracellular Ca2+ transients by confocal laser image analysis using fluo 3, and determined localization of Cx43 by immunofluorescence during in vitro differentiation of ES cells into cardiac myocytes. The transcripts for Cx43 and Cx45 were detected in undifferentiated ES cells and in embryoid bodies before and after the appearance of beating cardiomyocytes. In contrast, Cx40 transcripts were not observed in undifferentiated ES cells and were barely detectable in 3- and 5-day-old embryoid bodies. Cx40 transcripts significantly increased with the appearance of beating cells similar to those of cardiac-specific genes. Dye coupling was present among undifferentiated ES cells, prebeating cells of embryoid body outgrowth and ES cell-derived beating cardiomyocytes. When dye was injected into a beating cell, dye spread was restricted to neighboring beating cells. Immunofluorescence demonstrated that Cx43 protein was localized not only in beating cells but also in surrounding nonbeating cells, whereas myosin heavy chain alpha/beta was exclusively positive in the beating cells. These data suggest that the expression of multiple connexins is differentially regulated during the cardiomyocytic differentiation of ES cells in vitro and that Cx40 expression may be linked to early stages in cardiomyocytic differentiation.
Full text links
Related Resources
Trending Papers
Heart failure with preserved ejection fraction: diagnosis, risk assessment, and treatment.Clinical Research in Cardiology : Official Journal of the German Cardiac Society 2024 April 12
Proximal versus distal diuretics in congestive heart failure.Nephrology, Dialysis, Transplantation 2024 Februrary 30
World Health Organization and International Consensus Classification of eosinophilic disorders: 2024 update on diagnosis, risk stratification, and management.American Journal of Hematology 2024 March 30
Efficacy and safety of pharmacotherapy in chronic insomnia: A review of clinical guidelines and case reports.Mental Health Clinician 2023 October
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app