Immunolocalization of human gelatinase (type IV collagenase, MMP-9) and tissue inhibitor of metalloproteinase 1 in Hailey-Hailey and Darier's diseases.

BACKGROUND: The formation of lacunae and acantholysis as well as dyskeratosis are characteristic features of Hailey-Hailey disease (HHD) and Darier's disease (DD). Matrix metalloproteinases (MMPs) and their inhibitors like tissue inhibitors of metalloproteinases (TIMPs) have been thought to play major roles in the tissue metabolism.

OBJECTIVE: The aim of this study was to investigate the role of MMP-9 and TIMP-1 in HHD and DD.

METHODS: We examined localizations of these two molecules by immunostaining using specific monoclonal antibodies.

RESULTS: MMP-9 was positively stained in dyskeratotic or detaching cells around lacunae in HHD and DD. TIMP-1 showed a positive staining pattern throughout the epidermis.

CONCLUSION: MMP-9 might be involved in the pathophysiological process of HHD and DD in the presence of TIMP-1.