JOURNAL ARTICLE

The effect of dexamethasone on the in vitro incorporation of precursors of extracellular matrix components in the outflow pathway region of the rabbit eye

M R Hernandez, B I Weinstein, E J Wenk, G G Gordon, M W Dunn, A L Southren
Investigative Ophthalmology & Visual Science 1983, 24 (6): 704-9
6853096
The present study demonstrates that the cells of the outflow pathway region incorporate precursors of the extracellular matrix components during in vitro incubation. Addition of dexamethasone (10(-7) M) to the incubation mixture led to changes in precursor incorporation that were precursor and cell specific. The steroid significantly decreased the incorporation of 3H-glucosamine and increased the incorporation of 3H-proline in the outflow pathway cells and in the fibroblasts of the adjacent sclera. The specificity of the hormone effect was demonstrated by its inability to alter 3H-leucine incorporation. By contrast, in iridial fibroblasts dexamethasone caused a decreased incorporation of 3H-glucosamine while not affecting the incorporation of 3H-proline. Our results suggest that dexamethasone causes an increase in collagen synthesis in the target cells most closely related to the outflow pathway. Further, the decreased incorporation of 3H-glucosamine with dexamethasone suggests decreased synthesis of glycosaminoglycans, glycoproteins or possibly glycolipids in these same cells.

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