Selection of sucrose-dependent Escherichia coli to obtain envelope mutants and fragile cultures

G Mangiarotti, D Apirion, D Schlessinger
Science 1966 August 19, 153 (3738): 892-4
Mutants isolated as sucrose-dependent include many with apparent envelope defects, which fact often leads to filamentous growth or lysis in the absence of sucrose. One mutant can grow exponentially in 4 percent sucrose, but is very fragile: it releases all of its RNA and soluble protein when treated with 0.5 percent sodium deoxycholate. These characteristics permit the study of unstable structures and rapid processes in actively growing cells.

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