Add like
Add dislike
Add to saved papers

Multilocus sequence typing analysis and molecular characterization of carbapenemase related genes in Acinetobacter baumannii isolated from hospitalized patients in Erbil city, Iraq.

Acinetobacter baumannii, has been recognized by (WHO) as a global priority pathogen. It has been demonstrated to quickly pick up antimicrobial resistance genes. Multilocus sequence typing (MLST) is an unambiguous typing method for identifying accurate and portable nucleotide sequences of internal fragments of multiple housekeeping genes. The present study aimed to determine the sequence type using MLST, genetically define the carbapenem resistance phenotype and clarify the epidemiology of multidrug resistant (MDR) A. baumannii in the Kurdistan region of Iraq. Clinical samples were collected from ICU patients. VITEK 2 compact system was used for bacterial identification and antimicrobial susceptibility profile. PCR was used for detecting carbapenemase-related genes. Additionally, MLST was used to evaluate the genetic diversity of carbapenem-resistant isolates using Oxford scheme primers. In this investigation, 63 non-duplicate A. baumannii isolates from hospitalized patients were identified. According to CLSI standards, 75% and 73.4% of isolates were resistant to meropenem and imipenem respectively. Tigecycline and colistin were the most effective antibacterial agents. Of the various combinations of carbapenemase genes identified, the most common co-existence of genes present among clinical isolates were BlaOXA-23, and BlaOXA-51 (95.31%). While the less common combination of genes was detected in 4 isolates  (6.25 %) consisting of the co-existence of all tested carbapenemase genes in the present study (BlaOXA-23, BlaOXA-51, BlaOXA-58, BlaIMP, BlaVIM, BlaNDM). Regarding MLST analysis, the results confirmed that ST 556 (n=4) had the greatest frequency rate among clinical isolates, followed by ST 218 (n=3). Notably, the most common worldwide clonal lineage was CC92, which corresponded to (ICII). Only two isolates from ST 441, on the other hand, matched CC109/ ICI.  The present investigation revealed a significant level of diversity among A. baumannii isolates.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app