[Fertilization in vitro in the treatment of male infertility].

Thirty marital couples were included in an in vitro fertilization study of male infertility. Second-degree oligospermia (less than or equal to 5 X 10(6)/ml mobile sperms) was found in 3 couples, first-degree oligospermia (less than or equal to 12 X 10(6)/ml mobile sperms) in 21 couples, and asthenospermia (less than 20% of mobile sperms) in 6 couples. The semen for insemination was prepared by the standard procedure and the overlay technique. After the separation (removal) of seminal fluid and capacitation the sperms were found to be significantly more mobile. Ovarian cells obtained by laparoscopy were inseminated with 50,000 to 250,000 mobile sperms. The results were compared with those in a group of patients with normospermia (greater than or equal to 12 X 10(6)/ml mobile sperms). The number of fertilized ovarian cells proved to be significantly lower in patients with second-degree oligospermia (P less than 0.001). There was no difference in the rate of fertilization and ovarian cell division either in patients with first-degree oligospermia or asthenospermia or normospermia. Seven clinical pregnancies were recorded: 2 in the second-degree oligospermia group, 4 in the first-degree oligospermia group, and 1 in the asthenospermia group.