Distinct lipids profiles and associations with clinical indicators and gut microbiota in Prader-Willi syndrome children.

Lipid metabolism is tightly linked to adiposity. Prader-Willi syndrome (PWS) is a typical genetic disorder caused obesity, however, the distinct lipidomic profiles in PWS children have not been thoroughly investigated. Herein, serum lipidomics analysis were simultaneously explored in PWS, simple obesity (SO) and normal children (Normal). Results indicated that the total concentration of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) in PWS group were significantly deceased in comparison with both SO and Normal group. In contrast, compared with Normal group, there was an overall significantly increase on TAG level in both PWS and SO groups, with the highest was found in SO group. 39 and 50 differential lipid species were screened among three groups, and between obesity (PWS and SO) and Normal group, respectively. Correlation analysis revealed distinct profiles in PWS that different from other two groups. Notably, PC(P16:0/18:1), PE(P18:0-20:3), PE(P18:0-20:4)) showed significant negative correlation with Body mass index (BMI) soly in PWS groups. As for PE(P16:0-18:2), it showed negative association with BMI and weight in PWS group, but significant positive correlation in SO group, no statistical significant association were found in Normal group. We also found a significantly negative correlation between Blautia genus abundance and several significantly changed lipids, including LPC(14:0), LPC(16:0), TAG(C50:2/C51:9), TAG(C52:2/C53:9), TAG(C52:3/C53:10) and TAG(C52:4/C53:11), but no significant correlation in Normal group and SO group. Similarly, in PWS group, Neisseria genus was significantly negatively associated with CAR(14:1), CAR(18:0), PE(P18:0/20:3) and PE(P18:0/20:4), and extremely positively associated with TAG(C52:2/C53:9), while no obvious correlations were observed in Normal group and SO group.