The Effectiveness and Mechanical Properties of Chemotherapy-Impregnated Cement in Ewing Sarcoma.

Bone cement is often used in the surgical treatment of Ewing sarcoma (ES). Chemotherapy-impregnated cement (CIC) has never been tested in slowing ES growth. The purpose of the study is to determine if CIC can decrease cell proliferation, and to assess changes in the mechanical qualities of the cement. Chemotherapeutic agents including doxorubicin, cisplatin, etoposide, and SF2523 were mixed with bone cement. ES cells were plated and exposed to cell growth media that had contained CIC or regular bone cement (RBC) as a control, and cell proliferation assays were performed daily for three days. Mechanical testing on RBC and CIC was also performed. There was a significant decrease (p<0.001) in cell proliferation among all cells treated with CIC compared to cells treated with RBC by 48 hours post-exposure. Additionally, there was a synergistic effectiveness of the CIC noted when multiple antineoplastic agents were combined. Three-point-bending tests did not reveal substantial reductions in tolerated maximum bending load and maximal displacement at maximal bending load between CIC and RBC. This article is protected by copyright. All rights reserved.