In vivo efficacy of alcohol based hand rubs against noroviruses: A novel standardized European test method simulating practical conditions.

BACKGROUND: Non-enveloped viruses are particularly resistant to disinfectants, so to prevent and control the spread of virus infections, disinfectants with proven virucidal activity must be used. However, a test such as EN 1500, which uses an internal standard as reference treatment for determining the bactericidal efficacy of hand rubs, is still lacking. We aimed to establish a European standard for testing the in vivo efficacy of hand rubs against non-enveloped viruses.

METHOD: First, concentration and mode of application of ethanol as reference were determined. Afterwards, the efficacies of two commonly used hand rubs were compared to this reference. Hands of volunteers were contaminated with the murine norovirus strain S99.

RESULTS: 70% wt/wt ethanol (2 x 3 mL in 2 x 30 seconds) was defined as internal reference treatment. The commercial ethanol based hand rub could significantly reduce the titre of norovirus in 30 seconds whereas a rub based on ethanol and propan-2-ol was significantly less effective compared to the reference.

CONCLUSION: We have established a possible standard for testing the in vivo efficacy of hand rubs against non-enveloped viruses using murine norovirus, a low contamination volume technique and ethanol as internal reference. Nevertheless our findings have to be confirmed in European ring trials.