Glucose-activated switch regulating insulin analogue secretion enables long-term precise glucose control in type 1 diabetes mice.

We undertake this study to establish a glucose-responsive SIA self-supply system in vivo. Whether ER can serve as a safe and temporary repository to store designed fusion proteins and release SIAs under hyperglycemia condition for efficient blood glucose regulation. Intramuscularly expressed plasmid-encoded CAD-FCS-SIA fusion protein can be temporarily stored in ER and SIA can be released under the stimulation of hyperglycemia, resulting in efficient and long-term regulation of blood glucose stable in T1D mice. GAIS system provides applicable potential for type 1 diabetes therapy integrating regulation and monitor of blood glucose level.