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Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus.

Blenda Fernandes, Emanoelle Fernandes Rutren La Santrer, Sônia Maria de Figueiredo, Fabiana Rocha-Silva, Cláudia Barbosa Assunção, Amanda Gabrielle Abreu, Iara Furtado Santiago, Susana Johann, Rachel Basques Caligiorne
Revista da Sociedade Brasileira de Medicina Tropical 2023
BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR.

METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique.

RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated.

CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.

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