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Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system.

Wei Wang, Rui Bai, Xiantao Song
Stem Cell Research 2021 July 21
In order to determine the function of dHAND in a specific subset of cardiomyocyte progenitor cells responsible for the heart and its function in the cellular mechanism of Hand2-/- mouse ventricular hypoplasia, we established an allelic knockout model of dHAND in human embryonic stem cells (hESCs-H9) by an episomal vector-based CRISPR/Cas9 system. This dHAND KO hESC line maintained normal karyotype and typical primed pluripotent human stem cell morphology, and maintained pluripotency, could differentiate into all three germ layers in vivo.

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