We have located links that may give you full text access.
English Abstract
Journal Article
[Concentration of VEGF-A in the intraocular fluid of rats with alloxan model of diabetes mellitus].
Vestnik Oftalmologii 2021
PURPOSE: To study the changes in the concentration of vascular endothelial growth factor A (VEGF-A) in the intraocular fluid (IOF) of rats with alloxan model of diabetes mellitus (DM) on insulin therapy at different time points.
MATERIAL AND METHODS: The alloxan model of DM was simulated in 197 rats by a single intraperitoneal injection of 100 mg/kg alloxan hydrate. The animals were divided into 3 groups 7 days after administration of alloxan hydrate. The main group consisted of animals with alloxan model of DM, which begain receiving single daily intraperitoneal injections of insulin at a dose of 0.9 U/kg body weight. The comparison group included animals with alloxan model of DM, which did not receive the therapy. The control group consisted of healthy animals. The experimental animals were withdrawn from the study 1 and 4 months after the start of insulin therapy. The concentration of VEGF-A was determined in 80-90 μL of intraocular fluid collected from both eyes of each animal.
RESULTS: At 1 month, the VEGF-A concentration in the intraocular fluid in the study group ( n =17; 140 [136; 210] pg/mL) was statistically significantly higher than in the comparison group ( n =20; 72 [58; 86] pg/mL; p m-u <0.0004), and in the control group ( n =16; 76 [62.5; 88] pg/mL; p m-u =0.0045). The comparison group did not have statistically significant differences from the control group ( p m-u =0.9979). At 4 months, the VEGF-A concentration in the intraocular fluid in the study group ( n =18) was 84.8 [61.1; 93.2] pg/mL, in the comparison group ( n =16) - 66.4 [54.4; 73.75] pg/mL. The VEGF-A concentration in the intraocular fluid in the study group at 4 months was statistically significantly lower than in the study group at 1 month ( p m-u <0.0044).
CONCLUSION: Insulin therapy causes a statistically significant increase in the concentration of VEGF-A in the intraocular fluid of rats with alloxan model of DM after 1 month, but after 4 months of the therapy the VEGF-A concentration falls back to the initial values.
MATERIAL AND METHODS: The alloxan model of DM was simulated in 197 rats by a single intraperitoneal injection of 100 mg/kg alloxan hydrate. The animals were divided into 3 groups 7 days after administration of alloxan hydrate. The main group consisted of animals with alloxan model of DM, which begain receiving single daily intraperitoneal injections of insulin at a dose of 0.9 U/kg body weight. The comparison group included animals with alloxan model of DM, which did not receive the therapy. The control group consisted of healthy animals. The experimental animals were withdrawn from the study 1 and 4 months after the start of insulin therapy. The concentration of VEGF-A was determined in 80-90 μL of intraocular fluid collected from both eyes of each animal.
RESULTS: At 1 month, the VEGF-A concentration in the intraocular fluid in the study group ( n =17; 140 [136; 210] pg/mL) was statistically significantly higher than in the comparison group ( n =20; 72 [58; 86] pg/mL; p m-u <0.0004), and in the control group ( n =16; 76 [62.5; 88] pg/mL; p m-u =0.0045). The comparison group did not have statistically significant differences from the control group ( p m-u =0.9979). At 4 months, the VEGF-A concentration in the intraocular fluid in the study group ( n =18) was 84.8 [61.1; 93.2] pg/mL, in the comparison group ( n =16) - 66.4 [54.4; 73.75] pg/mL. The VEGF-A concentration in the intraocular fluid in the study group at 4 months was statistically significantly lower than in the study group at 1 month ( p m-u <0.0044).
CONCLUSION: Insulin therapy causes a statistically significant increase in the concentration of VEGF-A in the intraocular fluid of rats with alloxan model of DM after 1 month, but after 4 months of the therapy the VEGF-A concentration falls back to the initial values.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
A Guide to the Use of Vasopressors and Inotropes for Patients in Shock.Journal of Intensive Care Medicine 2024 April 14
Diagnosis and Management of Cardiac Sarcoidosis: A Scientific Statement From the American Heart Association.Circulation 2024 April 19
Essential thrombocythaemia: A contemporary approach with new drugs on the horizon.British Journal of Haematology 2024 April 9
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app