Transcriptome-wide N6-methyladenosine methylation landscape of coronary artery disease

Keyong Deng, Xiaotong Ning, Xiaoxiao Ren, Bin Yang, Jianxin Li, Jie Cao, Jichun Chen, Xiangfeng Lu, Shufeng Chen, Laiyuan Wang
Epigenomics 2021 April 20
Aim: To reveal transcriptome-wide N6-methyladenosine (m6 A) methylome of coronary artery disease (CAD). Materials & methods: The m6 A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m6 A methylome profiled by methylated RNA immunoprecipitation sequencing (MeRIP-seq) identified differentially methylated m6 A sites within both mRNAs and lncRNAs between CAD and control group. Results: Bioinformatic analysis indicated that differentially methylated genes were involved in the pathogenesis of atherosclerosis. MeRIP-quantitative real-time PCR assay confirmed the reliability of MeRIP-seq data. Finally, the rat carotid artery balloon injury model was performed to confirm the role of m6 A demethylase FTO in neointima formation. Conclusion: Our study provided a resource of differentially methylated m6 A profile for uncovering m6 A biological functions in the pathogenesis of CAD.

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