Macrophages expressing TREM-1 are involved in the progression of HPV16-related oropharyngeal squamous cell carcinoma.

INTRODUCTION: Many types of research have been performed to improve the diagnosis, therapy, and prognosis of oropharyngeal carcinomas (OP-SCCs). Since they arise in lymphoid-rich areas and intense lymphocytic infiltration has been related to a better prognosis, a TREM-1 putative function in tumour progression and survival has been hypothesized.

MATERIALS AND METHODS: Twenty-seven human papillomavirus (HPV) 16+ OP-SCC specimens have been analyzed to relate TREM-1 expression with histiocytic and lymphocytic markers, HPV presence and patients' outcome.

RESULTS: No differences have been shown between intratumoral and stromal CD4+ cells, while intratumoral CD8+ lymphocytes are higher with respect to the tumour stroma ( p   =   .0005). CD68+ cells are more than CD35+ and TREM-1+ ; their presence is related to CD35± and TREM-1± histiocytes ( p   =   .005 and .026, respectively). Intratumoral CD4+ lymphocytes are higher in p16+ cases (11/27) than in p16- ( p   =   .042); moreover, p16 positivity correlates to a better survival ( p   =   .034). CD4+ , CD8+ and CD35+ cells have no impact on survival, while CD68 expression heavily influences progression and bad outcome ( p   =   .037). TREM-1 positivity also leads to worst overall survival ( p   =   .001): peritumoral expression and death-cause relationship are always significant, particularly when the cause is OP-SCC ( p   =   .000).

CONCLUSION: While p16 shows to better stratify HPV16+ patients' outcome, TREM-1+ macrophages suggest their key importance in HPV-related OP-SCCs progression.KEY MESSAGESTREM-1 positivity correlates to the worst overall survival of HPV16-positive OPSCCs-affected patients.p16-positive HPV16 related OPSCCs patients have a better prognosis with respect to p16-negative ones.