We have located links that may give you full text access.
Use of a Multiplex Pcr Assay to Assess the Presence of TREPONEMA PALLIDUM in Mucocutaneous Ulcerations in Patients with Suspected Syphilis.
Journal of Clinical Microbiology 2020 November 12
We evaluated the utility of the commercial Allplex™ Genital Ulcer real-time PCR multiplex Assay for detecting Treponema pallidum (TPA), herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum; LGV) DNA in mucosal and genital ulcers in the context of suspected syphilis. In total, 374 documented genital and mucosal ulcers from patients with and without syphilis presenting at several sexually transmitted infection (STI) centers in France from October 2010 to December 2016 were analyzed at the National Reference Center for bacterial STIs at Cochin Hospital in Paris. TPA detection results were compared with the final diagnosis based on a combination of clinical examination, serological results and in-house nested PCR. Detections of HSV and LGV were validated against reference methods. We found that 44.6% of the 374 samples tested were positive for TPA, 21% for HSV, and 0.8% for LGV. No positive results were obtained for 30.7% of samples, and 4.8% presented co-infections. For TPA detection, the overall sensitivity was 80% (95% CI: 76.1-84.1%), specificity was 98.8% (95% CI: 97.7-99.9%), positive predictive value was 98.8% (95% CI: 97.7-99.9%) and negative predictive value was 80.2% (95% CI: 76.2-84.2%), with a rate of concordance with the reference method of 92.5% ( k = 0.85). This PCR multiplex assay is suitable for TPA detection in routine use and facilitates the simultaneous rapid detection of a broad panel of pathogens relevant in a context of suspected syphilis lesions.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
Perioperative echocardiographic strain analysis: what anesthesiologists should know.Canadian Journal of Anaesthesia 2024 April 11
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app