Etching Triangular Silver Nanoparticles by Self-generated Hydrogen Peroxide to Initiate Response of Electrochemiluminescence Sensing Platform.

This work outlines an electrochemiluminescence (ECL) platform with versatile and high-performance, which using the complex luminescent molecules [Ru(Ⅱ) complex] formed by carbohydrazide (CON4H6) and tris(4,4'-dicarboxylicacid-2,2'-bipyridyl)ruthenium(Ⅱ) dichloride [Ru(dcbpy)32+] as emitters to facilitate intramolecular ECL mechanism for reducing the response distance and interference, and they were immobilized on the porous bismuth vanadate nanoarray (BiVO4 NAs) to improve the orderliness of electron transfer. In addition, the detection was made depending on the self-generated hydrogen peroxide (H2O2) etching triangular silver nanoparticles (T-Ag NPs) to make ECL recovery response of the originally quenched due to ECL-RET between Ru(Ⅱ) complex (donor) and T-Ag NPs (receptor). Inspired by the antibac-terial application of dopamine, its own redox ability could produce more H2O2 for etching receptor T-Ag NPs under near-infrared (NIR) stimulation. Notably, in this system, the specific binding of antigens and antibodies with the autogenesis pro-cess of H2O2 and ECL detection procedure are independent. Therefore, the proposed system can avert the impact from complex biological samples effectively, and the ECL efficiency of the Ru(Ⅱ) complex can be readily utilized. On this basis, a biosensor is explored for primary diagnosis of squamous cell carcinoma by detecting the biomarker called after cytokeratin fragment 19 (CYFRA21-1), from which achieved an excellent linearity from 0.1 pg/mL to 50 ng/mL with detection limit of 0.058 pg/mL All of these results confirmed this strategy can be promising candidate for fabricating ECL-based biosensor.