Production of Donor-Derived Eggs After Ovarian Germ-Cell Transplantation into the Gonads of Adult, Germ Cell-Less, Triploid Hybrid Fish†

Dongdong Xu, Tasuku Yoshino, Marcelo Bello Cioffi, Hiroyuki Yoshikawa, Yasuko Ino, Ryosuke Yazawa, Samyra Maria Santos Nassif Lacerda, Yutaka Takeuchi
Biology of Reproduction 2020 September 17
In animals, spermatogonial transplantation in sterile adult males is widely developed; however, despite its utility, ovarian germ-cell transplantation is not well developed. We previously showed that the interspecific hybrid offspring of sciaenid was a suitable model for germ-cell transplantation studies as they have germ cell-less gonads. However, all these gonads have testis-like characteristics. Here, we tested whether triploidization in hybrid embryos could result in germ cell-less ovary development. Gonadal structure dimorphism and sex-specific gene expression patterns were examined in 6-mo-old triploid hybrids (3nHybs). Thirty-one percent of 3nHybs had germ cell-less gonads with an ovarian cavity. cyp19a1a and foxl2, ovarian differentiation-related genes, were expressed in these gonads, whereas dmrt1 and vasa were not expressed, suggesting ovary-like germ cell-less gonad development. Some (26%) 3nHybs had testis-like germ cell-less gonads. Ovarian germ cells collected from homozygous green fluorescent protein (GFP) transgenic blue drum (Nibea mitsukurii) were transplanted into 6-mo-old 3nHybs gonads via the urogenital papilla or oviduct. After 9 mo, the recipients were crossed with wild-type blue drum. Among the six 3nHyb recipients that survived, 1 female and 1 male produced fertile eggs and motile sperm carrying gfp-specific DNA sequences. Progeny tests revealed that all F1 offspring possessed gfp-specific DNA sequences, suggesting that these recipients produced only donor-derived eggs or sperm. Histological observation confirmed donor-derived gametogenesis in the 3nHyb recipients' gonads. Overall, triploidization reduces male-biased sex differentiation in germ cell-less gonads. We report, for the first time, donor-derived egg production in an animal via direct ovarian germ-cell transplantation into a germ cell-less ovary.

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