JOURNAL ARTICLE

Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, Hervé Acloque
Methods in Molecular Biology 2021, 2179: 341-351
32939732
Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to induce successive cycles of EMT/MET in an untransformed human mammary epithelial cell line (MCF10A) as well as the necessary controls for cycle validation.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
32939732
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"