Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition

Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P Girard, Nicolas Borghi
Methods in Molecular Biology 2021, 2179: 289-299
Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.

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