JOURNAL ARTICLE

Status of IL-4 and IL-10 driven markers in experimental models of Visceral Leishmaniasis

Srija Moulik, Joyshree Karmakar, Sumit Joshi, Anuradha Dube, Chitra Mandal, Mitali Chatterjee
Parasite Immunology 2020 July 31, : e12783
32734677

AIM: Leishmania donovani, the causative agent for visceral leishmaniasis (VL), modulates host monocytes-macrophages to ensure its survival. However, knowledge regarding the host-parasite interactions underpinning the disease remains limited. As disease progression is associated with polarization of monocytes/macrophages towards M2 which is regulated by cytokines IL-4/IL-13 and IL-10, this study evaluated the status of key IL-4 and IL-10 driven markers in experimental models of VL, as also evaluated their correlation, if any, with parasite load.

METHODS: In liver and splenic tissues from L.donovani infected hamsters and BALB/c mice, the parasite burden was determined along with mRNA expression of IL-4 driven markers i.e. CD206, Arginase-I, CCL17, CCL22, PPARγ , STAT-6,KLF-4, FIZZ1 and YM1 along with IL-10 driven markers, CXCL13, IL-10, TGFβ, VDR,CCR2 and CYP27A1.

RESULTS: The mRNA expression of IL-4 and IL-10 driven markers was enhanced in both models, but only in the hamster model, the splenic tissues demonstrated a positive correlation between all the IL-10 driven markers and parasite load.

CONCLUSIONS: Contrary to human VL, both models demonstrated an increased expression of IL-4 and IL-10 driven markers.

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