Remote Ischemic Postconditioning Inhibits Hippocampal Neuronal Apoptosis and Mitophagy After Cardiopulmonary Resuscitation in Rats

Biao Xie, XuHui Gao, Yang Huang, Yu Zhang, Shuibo Zhu
Shock 2020 June 23

BACKGROUND: Studies have shown that remote ischemic postconditioning can improve brain damage caused by ischemia and hypoxia. However, the specific mechanism underlying this phenomenon is still unclear. The purpose of this study was to investigate the effects of remote ischemic postconditioning on neuronal apoptosis and mitophagy after cardiopulmonary resuscitation (CPR) in rats.

METHODS: Male Sprague-Dawley rats were used to establish an asphyxia cardiac arrest model by clamping the tracheal duct. Firstly, the expression levels of P53, Cytochrome c (Cytc), and Parkin in the cytoplasm and mitochondria were observed at 3, 6, 24, and 72 hours after the restoration of spontaneous circulation (ROSC). Then, neurological deficit scores (NDS), hippocampal neuron apoptosis, mitochondrial P53 and Parkin, cytoplasmic Cytc, and neuron ultrastructure were evaluated 24 hours after ROSC.

RESULTS: P53 and Parkin can translocate from the cytoplasm to the mitochondria, promoting the translocation of cytoplasmic Cytc to mitochondria after CPR, reaching a peak at 24 hours after the ROSC. The P53 inhibitor Pifithrin-μ reduced apoptosis induced by P53 mitochondrial translocation. Apoptosis was induced after cardiac arrest (CA) and attenuated by remote ischemic postconditioning via inhibiting P53 mitochondrial translocation and the release of Cytc to the cytoplasm. In addition, remote ischemic postconditioning could inhibit Parkin-mediated mitophagy.

CONCLUSION: Taken together, our results show that remote ischemic postconditioning improves neural function after CPR by inhibiting P53 mitochondrial translocation-induced apoptosis and Parkin-mediated mitophagy.

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