Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses

Daisuke Oikawa, Yusuke Sato, Fumiaki Ohtake, Keidai Komakura, Kazuki Hanada, Koji Sugawara, Seigo Terawaki, Yukari Mizukami, Hoang T Phuong, Kiyosei Iio, Shingo Obika, Masaya Fukushi, Takashi Irie, Daisuke Tsuruta, Shinji Sakamoto, Keiji Tanaka, Yasushi Saeki, Shuya Fukai, Fuminori Tokunaga
Communications Biology 2020 April 3, 3 (1): 163
The NF-κB and interferon antiviral signaling pathways play pivotal roles in inflammatory and innate immune responses. The LUBAC ubiquitin ligase complex, composed of the HOIP, HOIL-1L, and SHARPIN subunits, activates the canonical NF-κB pathway through Met1-linked linear ubiquitination. We identified small-molecule chemical inhibitors of LUBAC, HOIPIN-1 and HOIPIN-8. Here we show that HOIPINs down-regulate not only the proinflammatory cytokine-induced canonical NF-κB pathway, but also various pathogen-associated molecular pattern-induced antiviral pathways. Structural analyses indicated that HOIPINs inhibit the RING-HECT-hybrid reaction in HOIP by modifying the active Cys885, and residues in the C-terminal LDD domain, such as Arg935 and Asp936, facilitate the binding of HOIPINs to LUBAC. HOIPINs effectively induce cell death in activated B cell-like diffuse large B cell lymphoma cells, and alleviate imiquimod-induced psoriasis in model mice. These results reveal the molecular and cellular bases of LUBAC inhibition by HOIPINs, and demonstrate their potential therapeutic uses.

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