JOURNAL ARTICLE

Agaphelin modulates the activation of human bronchial epithelial cells induced by lipopolysaccharide and IL-4

Daniely Cornélio Favarin, Aline Beatriz Mahler Pereira, Ivo M B Francischetti, Marcos Vinicius da Silva, Virmondes Rodrigues, Paulo Roberto da Silva, Jesus G Valenzuela, David Nascimento Silva Teixeira, Carlo José Freire Oliveira, Alexandre de Paula Rogério
Immunobiology 2020 March 18, : 151937
32201094
Sand fly saliva presents molecules with potential to development of compounds for treatment of inflammatory diseases. Agaphelin, isolated from the saliva of the mosquito Anopheles gambiae, demonstrates anti-inflammatory properties such as neutrophils chemotaxis inhibition. Here, we extend these results and evaluated the role of agaphelin (0.1-100 nM) in an in vitro model consisting in the activation of human bronchial epithelial cells (BEAS-2B) by IL-4 (50 ng/mL) or lipopolysaccharide (LPS; 10 ng/mL). Agaphelin is non-cytotoxic for BEAS-2B cells. Notably, agaphelin markedly reduces CCL2 and IL-8 production induced by IL-4 or LPS, without altering the IL-10 production. The TLR4 expression and STAT1 phosphorylation induced by LPS were inhibited by agaphlin. In addition, agaphelin decreased the phosphorylation of STAT6 induce by IL-4, whose effect was independent of IL-4-binding activity. Taken together, these findings identify agaphelin as a potential anti-inflammatory therapeutic agent for airway inflammations.

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