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Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies.

Nisreen M A Okba, Ivy Widjaja, Wentao Li, Corine H GeurtsvanKessel, Elmoubasher A B A Farag, Mohammed Al-Hajri, Wan Beom Park, Myoung-Don Oh, Chantal B E M Reusken, Marion P G Koopmans, Berend-Jan Bosch, Bart L Haagmans
Emerging Infectious Diseases 2020 May
We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.

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