Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews

A S Garcez, L C Barros, M R U Fernandes, D N Fujii, S S Suzuki, R Nepomuceno
Photodiagnosis and Photodynamic Therapy 2020 February 29, : 101707

INTRODUCTION: Peri-miniscrew inflammation is one of the causes of orthodontic miniscrew failure.

OBJECTIVE: The aim of this study was to correlate the quantification throughout autofluorescence images and PCR microbiologic count of biofilm retained around orthodontic miniscrew and the presence of Porphyromonas gingivalis.

MATERIALS AND METHODS: Forty miniscrews used for orthodontic treatment were evaluated during orthodontic treatment, collected from patients and divided into two groups: health and inflamed miniscrews. To be considered inflamed, the samples should present: loss of stability checked by periotest®, clinical presence of mucositis, red aspect of the gum or bleeding around the miniscrew. Immediately after removal of the miniscrews, they were photographed using a macro 100 lens and a Pentax camera coupled to a fluorescent equipment - Qscan (AioBio - Korea) with 405 nm excitation wavelength and a blue band filter. A microbiologic sample was collected with a sterile microbrush scrubbed around the miniscrew tread. Fluorescent images were analyzed with ImageJ software to quantify fluorescent intensity and fluorescent area and microbiological samples were submitted to UCF count for total contamination and q-PCR assay to quantify Porphyromonas gingivalis.

RESULTS: The results showed a good correlation between UFC count and fluorescent intensity and PCR/fluorescent area. The health miniscrews presented less fluorescent intensity and lower UFC count when compared to inflamed miniscrews. q-PCR analysis showed a higher number of P. gingivalis contamination around inflamed miniscrews.

CONCLUSION: Quantification of biofilm retained by miniscrew by images of auto-fluorescence is a simple and reliable method with great potential for clinical use to monitory inflammation around miniscrew and risk of loss.

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