Design of selegiline loaded Bio-nanosuspension for the management of depression using novel Bio-retardant from Manilkara zapota.

BACKGROUND: Depression is one of the most frequent psychiatric and potentially life-threatening disorders. This research work can offers a potential for delivery of selegiline moiety via ocular route in bio-nanosuspension mode for the effective management of depression after preclinical performance screening.

RESEARCH DESIGN AND METHODS: The selegiline loaded Bio-nanosuspension was prepared using novel bio-retardant isolated from fruit pulp of Manilkara zapota (Sapodilla) by sonication solvent evaporation method with different ratios (0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 1%) and with standard polymer HPMC (0.1%, 0.2%, 0.3%, 0.4%, 0.5%). The prepared formulations were evaluated for pH stability studies, %entrapment efficacy, in-vitro drug release, particle size, polydispersity index, zeta potential, stability studies.

RESULTS: The prepared bio-nanosuspension was subjected to the best formulation based on comparison of above mentioned evaluation parameters, so Fb2 (0.1%) formulation was found to be the best formulation showing an R2 value of 0.9814, T50% of 29.7 hrs and T80% of 65.25 hrs respectively. According to the release kinetics the best fit model was found to be Peppas Korsmeyer with Fickian Diffusion (Higuchi Matrix) as the mechanism of drug release.Manilkara zapota (Sapodilla) provided excellent stability for the formulation and resulting particle size for the best formulation was found to be 252 nm.The bio-nanosuspension had Polydispersity Index (PDI) of 0.35 with zeta potential of -8.91 mV.

CONCLUSION: The prepared bio-nanosuspension was found to be safe and compatible with the ophthalmic delivery for treatment of depression.