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The Cystathionine beta-lyase is crucial for embryo patterning and the maintenance of root stem cell niche in Arabidopsis.

Plant Journal 2019 April 20
The growth and development of roots in plants depends on the specification and maintenance of the root apical meristem. Here, we report the identification of CBL, a gene required for embryo and root development in Arabidopsis, and encodes cystathionine beta-lyase (CBL) that catalyzes the penultimate step in methionine (Met) biosynthesis, which also led to the elucidation of a previous unknown but crucial metabolic contribution by the Met biosynthesis pathway. CBL is expressed in embryos and showing quiescent center (QC)-enriched expression pattern in the root. cbl mutant has impaired embryo patterning, defective root stem cell niche, stunted root growth, and reduces accumulation of the root master regulators PLETHORA1 (PLT1) and PLT2. Furthermore, mutation in CBL severely decreases abundance of several PIN-FORMED (PIN) proteins and impairs auxin-responsive gene expression in the root tip. cbl seedlings also exhibit global reduction of histone H3 Lys-4 trimethylation(H3K4me3)and DNA methylation.Importantly, mutation in CBL reduces the abundance of H3K4me3 modification in PLT1/2 genes and down-regulates their expression. Overexpression of PLT2 partially rescues cbl root meristem defect,suggesting that CBL acts in part through PLT1/2. Moreover, exogenous supplementation of Met also restores the impaired QC activity and the root growth defects of cbl. Taken together, our results highlight the unique role of CBL to maintain the root stem cell niche by cooperative actions between Met biosynthesis and epigenetic modification of key developmental regulators. This article is protected by copyright. All rights reserved.

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