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JOURNAL ARTICLE
REVIEW
A CRISPR/Cas9 based polymeric nanoparticles to treat/inhibit microbial infections.
Seminars in Cell & Developmental Biology 2019 April 13
The latest breakthrough towards the adequate and decisive methods of gene editing tools provided by CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR Associated System), has been repurposed into a tool for genetically engineering eukaryotic cells and now considered as major innovation in gene related disorders. Nanotechnology has provided an alternate way to overcome the conventional problems where methods to deliver therapeutic agents have failed. The use of nanotechnology has the potential to safe-side the CRISPR/Cas9 components delivery by using customized polymeric nanoparticles for safety and efficacy. The pairing of two (CRISPR/Cas9 and nanotechnology) has the potential for opening new avenues in therapeutic use. In this review, we will discuss the most recent advances in developing nanoparticle-based CRISPR/Cas9 gene-editing cargo delivery with focus on several polymeric nanoparticles including fabrication proposals to combat microbial infections.
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