Shortwave Near-Infrared Spectroscopy for Rapid Detection of Aflatoxin B 1 Contamination in Polished Rice.

The objective of this research was to apply near-infrared spectroscopy, with a short-wavelength range of 950 to 1,650 nm, for the rapid detection of aflatoxin B1 (AFB1 ) contamination in polished rice samples. Spectra were obtained by reflection mode for 105 rice samples: 90 samples naturally contaminated with AFB1 and 15 samples artificially contaminated with AFB1 . Quantitative calibration models to detect AFB1 were developed using the original and pretreated absorbance spectra in conjunction with partial least squares regression with prediction testing and full cross-validation. The statistical model from the external validation process developed from the treated spectra (standard normal variate and detrending) was most accurate for prediction, with a correlation coefficient ( r) of 0.952, a standard error of prediction of 3.362 μg/kg, and a bias of -0.778 μg/kg. The most predictive models according to full cross-validation were developed from the multiplicative scatter correction pretreated spectra ( r = 0.967, root mean square error in cross-validation [RMSECV] = 2.689 μg/kg, bias = 0.015 μg/kg) and standard normal variate pretreated spectra ( r = 0.966, RMSECV = 2.691 μg/kg, bias = 0.008 μg/kg). A classification-based partial least squares discriminant analysis model of AFB1 contamination classified the samples with 90% accuracy. The results indicate that the near-infrared spectroscopy technique is potentially useful for screening polished rice samples for AFB1 contamination.