EWOD Silicon Biosensor for Multiple Nucleic Acids Analysis

Salvatore Petralia, Daniele Motta, Sabrina Conoci
Biotechnology and Bioengineering 2019 April 9
In this paper, a miniaturized biosensor containing 96 silicon micro-chambers electro-loaded with nano-volumes of liquid (EW-Chip) is presented. The liquid electro-loading is achieved by the appropriate modulation of interface properties. The surface chemistries have been studied to guarantee effective interface properties for both ElectroWetting on Dielectric (EWoD) actuaction and biocompatibility versus biochemical reactions. The silicon micro-chambers are 200 nl in volume and are connected to a specific system of electrodes able to deliver liquid sample on each wells. The device also integrates temperature sensors and heaters to perform biochemical reactions. On that, the effectiveness of this device has been successfully proven towards the nucleic acids detection via real time PCR amplification. HBV (Hepatitis B Virus) genome target has been used to assess the device performance. Results show very uniform amplification over the 96 micro-chambers without any cross-contamination process. These features make this system a very appealing potential solution for genetic point-of-care deices where high level of parallelism of analysis is required. This article is protected by copyright. All rights reserved.

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