Read by QxMD icon Read

Preparation and characterization of human scar acellular dermal matrix

Chenzhi Lai, Guodong Song, Bo Zhao, Hongquan Wang, Bo Pan, Xiaoshuang Guo, Xiaolei Jin, Xianlei Zong
Journal of Biomaterials Science. Polymer Edition 2019 April 5, : 1-14

INTRODUCTION: Acellular dermal matrix (ADM) is commonly used to treat burn injuries and wounds. In this study, we aimed to analyze the structural and biomechanical characteristics of an ADM from human scars.

METHODS: We randomly selected human mature scars, human proliferative scars, and human normal skin as experimental specimens. Split-thickness dermal grafts were obtained using a free-hand graft knife. Samples were subjected to cell lysis to obtain ADMs. Structural analysis was performed via light microscopy, fluorescence microplate reader and scanning electron microscopy. Thereafter, human epithelial stem cells were incubated on these ADMs. Finally, the biomechanical characteristics of the ADMs were analyzed using a tensile machine.

RESULTS: Normal skin ADM fibers were arranged in order, whereas proliferative scar ADM fibers were dense and disordered, and mature scar ADM fibers were porous and slightly disordered. The residual DNA of three ADM meet the residual DNA standard of biological material. After incubating human epithelial stem cells on ADMs, cells grew in an aggregated state in both normal skin ADMs and mature scar ADMs; however, cells adhered only on the surface of proliferative scar ADMs. No significant differences were observed in the Young's modulus, relaxation slope, creep slope, creep, or maximum tensile stress among the three ADMs, although significant differences in stress-strain elongation and relaxation were noted.

CONCLUSIONS: Our findings showed that mature scar ADMs were similar to proliferative scar ADMs, showing a slight lag compared with normal skin ADMs, providing insights into the biomechanical features of these scar tissues.


You need to log in or sign up for an account to be able to comment.

No comments yet, be the first to post one!

Trending on Read

Available on the App Store

Available on the Play Store
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"