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Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection

Meltem Koca Esentürk, Semra Akgönüllü, Fatma Yılmaz, Adil Denizli
Journal of Biomaterials Science. Polymer Edition 2019 March 28, : 1-13
Human serum albumin (HSA) is a major blood plasma protein also found in urine where its existence may be a marker of some types of liver or kidney dysfunction. Herein, we fabricated a novel surface plasmon resonance (SPR) nanosensor for selective, sensitive, and label-free microalbumin detection both in aqueous and urine sample solutions. Firstly, HSA-imprinted nanoparticles were synthesized, which consist of ethylene glycol dimethacrylate and N-methacryloyl-L-leucine as a cross-linker and functional monomer. The nanoparticles were characterized by zeta-size and scanning electron microscope analyses and were dropped onto the SPR chip surface to make HSA sensitive nanosensor. Characterization studies of HSA-imprinted SPR chip were carried out by atomic force microscopy, Fourier-transform infrared spectroscopy, contact angle, and ellipsometer. The limit of detection and limit of quantification values of HSA-imprinted SPR nanosensor were calculated as 0.7 pM and 1.9 pM for the concentration range of 0.15-500 nM. Selectivity studies of HSA-imprinted SPR nanosensor were achieved with hemoglobin and transferrin proteins which were chosen as competitor molecules. HSA-imprinted SPR nanosensor was displayed highly selective and sensitive to HSA. Highlights A molecularly imprinted based SPR nanosensor shows perfect selectivity and sensitivity of microalbumin. HSA-imprinted SPR nanosensors enable to monitor HSA in real-time for kinetic rate constants determination. HSA-imprinted SPR nanosensors can be a cost-effective solution due to the reproducibility.


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