Add like
Add dislike
Add to saved papers

Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology.

Proteomics 2019 March 28
The use of cultured cells has been instrumental in studying biochemical, molecular, and cellular processes. The composition of serum that cells are maintained in can have a profound impact on important cellular checkpoints. We have analyzed cell growth and apoptosis in an estrogen receptor positive breast cancer cell line in the presence of serum that have been treated to remove steroids or lipids, as well-described in the literature. We showed that maintaining cells in the presence of charcoal-dextran-treated serum causes reduced growth rate, which can be reversed by the addition of estradiol. Silica-treated-serum also slowed down cell growth and induced apoptosis. In order to investigate the role of lipids in these phenotypes, we investigated the levels of a wide range of lipids in different sera. We showed that silica-treatment significantly depletes phosphatidylcholines and cholesterol. We also show that lipogenesis is stimulated when cells are cultured with silica-treated-serum and this was reversed by the addition of exogenous lipids, which also restored growth rate and apoptosis. Our results show that cultured cells are sensitive to different serum, most likely due to the differences in levels of structural and signaling metabolites present in their growth environment. This article is protected by copyright. All rights reserved.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app