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The chromatin-associated protein PWO1 interacts with plant nuclear lamin-like components to regulate nuclear size

Pawel Mikulski, Mareike L Hohenstatt, Sara Farrona, Cezary Smaczniak, Yvonne Stahl, Kalyanikrishna Kalyanikrishna, Kerstin Kaufmann, Gerco C Angenent, Daniel Schubert
Plant Cell 2019 March 26
Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). NL is a protein mesh that resides underneath the inner nuclear membrane (INM) and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast to animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between lamin-like protein, CROWDED NUCLEI 1 (CRWN1), and the chromatin- and PcG-associated component, PWWP INTERACTOR OF POLYCOMBS 1 (PWO1), in Arabidopsis thaliana. We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology and control expression of a similar set of target genes. Moreover, we demonstrate that PWO1 proteins forms foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and nuclear lamina.


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