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Anti-adipogenesis and antioxidative defense status of Kalasin 2 cultivar peanut sprout crude extract in 3T3-L1 mouse adipocytes.
Biochemistry and Cell Biology 2019 March 26
The aim of this study was to investigate the effects of germinated (GPE) and non-germinated peanut (NGPE) extracts on adipogenesis and oxidative status in normal and oxidative stress-induced 3T3-L1 mouse adipocytes. The treated cells were analysed for cell growth, lipid accumulation, intracellular ROS and the expression of mRNAs and proteins related to adipogenesis and antioxidative defense systems. The results indicated that 9 day-germinated peanut extract (9GPE) reduced lipid contents and mRNA expression of adipogenesis-related genes greater than 1-day germinated (1GPE) and NGPE, respectively. In oxidative stress-induced adipocytes, 9GPE decreased ROS levels, lipid contents, and peroxisome proliferator-activated receptor gamma protein expression as well as increased antioxidant protein expression. These results show the anti-adipogenic capacity and oxidative status improvement of GPE and it could be used as a putative therapeutic agent in the prevention of and/or treatment of obesity and oxidative-relative diseases.
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