Fibro-Neuronal Guidance on Common, 3D-Printed Textured Substrates.

Ability to direct neuronal growth not only carries great potential for treating neural conditions -e.g. bridging traumatically shattered connections - but would also be an exquisite tool for bionic applications that require physical interface between neurons and electronics. A testing platform is needed to better understand axonal guidance in the context of a specific in-vivo application. Versatility of 3D printing technology allows tailoring to researcher needs, both in-vitro and in-vivo. In this study we establish a fibro-neuronal co-culture inspired by our neural interface research, and demonstrate axon alignment on a texturized substrate fabricated with a common, versatile 3D-printing set-up.